Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium komossense Kazda and Muller 1979.
Acid-alcohol-fast, short to moderately long rods, often clumped. No cording. No
cross-barring. Gram-positive. Spores, capsules, true branching, and aerial hyphae
are not produced.
Colonies on Lowenstein-Jensen medium are eugonic, smooth, glistening,
yellow-beige-pigmented when incubated between 22 and 37 ºC. At 31 ºC, growth
is observed after 3 days; at 22 and 37 ºC, it is observed after 7 days. Dilute inocula on
Middlebrook agar yields smooth, compact, glistening, yellow-beige colonies 0.5 to 2
mm in diameter and with entire margins. No growth at 45 ºC. No growth on
MacConkey agar or on media supplemented with 5% NaCl.
Isolated from sphagnum vegetation of intact sphagnum bogs in southern Sweden and the Atlantic coastal area of Norway; not
recovered from partially cultivated moors.
Susceptible to isoniazid (1 μg/ml). Resistant to to hydroxylamine (500 μg/ml). Variable resistance to ethambutol.
Injection of guinea pigs, mice, and rabbits with large inocula (1-10 mg wet weight of cells) produced neither local nor disseminated
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Kazda J, Muller K. Mycobacterium komossense sp. nov. International Journal of Systematic Bacteriology 1979; 29:361-365.
- Tsukamura M, Mizuno S, Tsukamura S. Numerical analysis of rapidly growing, scotochromogenic mycobacteria, including
Mycobacterium obuense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov.,
nom. rev., Mycobacterium chubuense sp. nov., nom. rev., and Mycobacterium tokaiense sp. nov., nom. rev. Int. J. Syst. Bacteriol.
- Vuorio R, Andersson MA, Rainey FA, Kroppenstedt RM, Kampfer P, Busse HJ, Viljanen M, Salkinoja-Salonen M. A new rapidly
growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre. Int
J Syst Bacteriol 1999; 49:25-35.
Positive results for acid phosphatase, arylsulphatase (14 days), alpha- and beta-esterase, iron uptake, succinamidase, Tween
hydrolysis (5 days), and urease.
Acid is produced from glucose, fructose, D-mannitol, rhamnose, sorbitol, and D-trehalose.
Can utilize as sole carbon source fumarate, succinate, citrate, oxalate, hippurate, ethanol, and propanol.
Negative results for arylsulphatase (3 days), semi-quantitative catalase test, beta-galactosidase, nitrate reduction, niacin,
acetamidase, benzamidase, isonicotinamidase, and.
No utilization as sole carbon source of benzoate, and sucrose.
Variable results for thermostable catalase (68 ºC), malonamidase, nicotinamidase, pyrazinamidase, utilization of malonate.
(c) Costin Stoica