Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium immunogenum Wilson et al. 2001.
Acid-alcohol-fast, curved rods. Do not form spores or aerial hyphae. Gram-positive.
Colonies arenon-pigmented, off-white in color, and rough, although smooth forms
may occur. Visible growth appears on Middlebrook 7H10 and trypticase soy agars in
less than 7 days. Growth occurs at 24, 30, 35, 37 ºC, but not at 45 ºC. Growth occurs
on MacConkey agar (without crystal violet), but not in media supplemented with 5%
Isolated from a sample of bronchoscope wash water, other clinical samples, metalworking fluids.
Susceptible in vitro to amikacin and clarithromycin. Resistant to cefoxitin, cefmetazole, ciprofloxacin, doxycycline, imipenem,
sulfamethoxazole and tobramycin.
Involved in water-borne nosocomial outbreaks along with strains of Mycobacterium abscessus and Mycobacterium chelonae.
Identified as a cause of keratitis following laser in situ keratomileusis. Involved in in hypersensitivity pneumonitis associated
with metalworking fluid. Cutaneous infection reported (erythematous inflammatory papules and pustules on the abdomen).
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Wilson RW, Steingrube VA, Bottger EC, Springer B, Brown-Elliott BA, Vincent V, Jost KCJ, Zhang Y, Garcia MJ, Chiu SH, et al.
Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical
disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy.
Int J Syst Evol Microbiol 2001; 51:1751-1764.
- Adekambi T, Reynaud-Gaubert M, Greub G, Gevaudan MJ, La Scola B, Raoult D, Drancourt M. Amoebal coculture of
"Mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumonia. J Clin Microbiol 2004; 42:5493-
- Garcia-Zamora E, Sanz-Robles H, Elosua-Gonzalez M, Rodriguez-Vasquez X, Lopez-Estebaranz JL. Cutaneous infection due to
Mycobacterium immunogenum: an European case report and review of the literature. Dermatol Online J. 2017;23(10):
13030/qt9zg5r07t. Published 2017 Oct 15.
- Nogueira CL, Whipps CM, Matsumoto CK, Chimara E, Droz S, Tortoli E, de Freitas D, Cnockaert M, Palomino JC, Martin A, et al.
Mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium
chelonae--Mycobacterium abscessus group. Int J Syst Evol Microbiol 2015; 65:4403-4409.
Positive results for alkaline phosphatase, arylsulfatase (3 days), arginine dihydrolase, catalase, semiquantitative catalase test,
esculin hydrolysis, beta-galactosidase, alpha-glucosidase, lysine decarboxylase, ornithine decarboxylase, pyrrolidonyl arylamidase
Negative results for iron uptake, N-acetyl beta-glucosaminidase, beta-glucuronidase, gelatinase, indole production, nitrate reduction,
Tween 80 hydrolysis, urease, and acetoin production.
No utilization as sole carbon source of citrate, mannitol, D-sorbitol, and myo-inositol.
Variable results for pyrazinamidase.
(c) Costin Stoica