Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium holsaticum Richter et al. 2002.
Acid-alcohol-fast, short or coccoid rods.
Colonies are dysgonic and transparent at 22 ºC, but smooth, moist, shiny and off-
white- to yellow-pigmented at higher temperatures. Isolates grow within 7 days at
temperatures up to 40 ºC; higher temperatures seem to favor growth. No growth at 45
ºC. Growth occurs on media supplemented with 5% (w/v) NaCl, but not on MacConkey
agar (without crystal violet) .
Isolated from clinical specimens (sputum, urine, gastric fluid). Resistant to thiophene-2-carboxylic acid hydrazide (2 µg/ml), isoniazid
and rifampin, but susceptible to streptomycin, ethambutol, protionamide and ofloxacin.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Richter E, Niemann S, Gloeckner FO, Pfyffer GE, Rüsch-Gerdes S. Mycobacterium holsaticum sp. nov. Int J Syst Evol Microbiol.
2002;52(Pt 6):1991-1996. doi:10.1099/00207713-52-6-1991
Positive results for acid phosphatase, nicotinamidase, pyrazinamidase, nitrate reduction, Tween 80 hydrolysis (weak reaction), and
Negative results for arylsulfatase, acetamidase, benzamidase, allantoinamidase, succinamidase, heat-stable catalase, iron uptake,
tellurite reduction (3 days).
(c) Costin Stoica