Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium hiberniae Kazda et al. 1993.
Member of the Mycobacterium terrae complex.
Acid-alcohol-fast, beaded rods, 0.9 x 1.2-1.5 μm. Frequently forms clumps. Not
forming cords, aerial hyphae, capsules, spores, or true branching.
Colonies on Lowenstein-Jensen and Middlebrook 7H10 agar, after incubation for 20
days are smooth, compact, and glistening rose-pink, 1-1.5 mm in diameter. Eugonic
growth in the first phase, but later become dysgonic and assume a rough, dry
appearance after longer incubation (8 weeks). Temperature range for growth is 22-37
ºC; optimal growth is at 37 ºC; does not grow at 42 or 45 ºC. No growth in media
supplemented in 5% (w/v) NaCl.
Isolated from moss (Ctenidium molluscum), sphagnum vegetation, and soil collected in counties Clare and Donegal in Ireland.
Susceptible to ethambutol (2 µg/ml). Resistant to isoniazid (1 µg/ml), (variable), streptomycin (5 µg/ml), ethionamide (10 and 40
µg/ml), rifampin (20 µg/ml), and potassium tellurite.
Not pathogenic for guinea pigs, mice, or rabbits. Produce a non-specific hypersensitivity reaction to bovine tuberculin in guinea pigs
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Kazda J, Cooney R, Monaghan M, Quinn PJ, Stackebrandt E, Dorsch M, Daffe M, Muller K, Cook BR, Tarnok ZS. Mycobacterium
hiberniae sp. nov. Int J Syst Bacteriol 1993; 43:352-357.
- Jamal Saad, Michael Phelippeau, May Khoder, Marc Levy, Didier Musso & Michel Drancourt. “Mycobacterium mephinesia”, a
Mycobacterium terrae complex species of clinical interest isolated in French Polynesia. Scientific Reports | (2019) 9:11169 | https:
- Neonakis IK, Spandidos DA, Gitti Z. Mycobacterium heraklionense sp. nov.: A case series. Exp Ther Med. 2015;10(4):1401-1403.
- Masaki T, Ohkusu K, Hata H, Fujiwara N, Iihara H, Yamada-Noda M, Nhung PH, Hayashi M, Asano Y, Kawamura Y, et al.
Mycobacterium kumamotonense Sp. Nov. recovered from clinical specimen and the first isolation report of Mycobacterium
arupense in Japan: Novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex. Microbiol
Immunol 2006; 50:889-897.
Positive results for acid phosphatase, arylsulfatase (10 days), catalase, beta-galactosidase, and nitrate reduction.
Negative results for arylsulfatase (3 days),beta-glucosidase, niacin production, allantoinase, benzamidase, isonicotinamidase,
nicotinamidase, pyrazinamidase (positive in Saad's paper - API test), succinamidase, urease, acid production from glucose and
Variable results for Tween 80 hydrolysis.
(c) Costin Stoica