Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium helvum Tran and Dahl 2016.
Acid-fast coccoid rods. Older cultures show a decrease in acid-fastness.
Colonies are pale yellow, scotochromogenic, shiny, smooth with a slightly ridged
margin. Growth on Middlebrook 7H11 agar with oleic, albumin, dextrose, catalase
reaches maturity in 7 days at 28 ºC and in 5 days at 37 ºC. Can grow in 5% NaCl
media. Can survive at 8 ºC with little cell replication.
Isolated from water samples collected from S. purpurea pitchers at the Laurentian Educational Center in northern Minnesota.
Susceptible to chloramphenicol, ciprofloxacin, and streptomycin. Resistant to penicillin, aztreonam, and trimethoprim.
- Tran PM, Dahl JL. Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., isolated from the pitcher plant
Sarracenia purpurea. Int J Syst Evol Microbiol 2016; 66:4480-4485.
Positive results for arylsulfatase (3 and 14 days) and nitrate reductase.
Negative results for catalase, heat-stable catalase, tellurite reduction, Tween 80 hydrolysis and urease.
(c) Costin Stoica