Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium haemophilum Sompolinsky et al. 1978.
Strongly acid–alcohol-fast, short (1.4–3.2 x 0.4–0.7 μm), occasionally curved, rods,
occurring singly or in minor cordlike formations. Not stainable by the Gram method.
Colonies after incubation for 2-4 weeks on Middlebrook 7H10 agar or Lowenstein-
Jensen medium are rough and non-pigmented. Small numbers of smooth colonies
often occur. Growth requires supplementation with 0.4% hemoglobin, 60 μM hemin or
15 mg/ml ferric ammonium citrate. Temperature range for growth is 25-35 ºC and
optimal growth is at 30-32 ºC; does not grow at 37, 42 or 45 ºC. Does not grow on
media supplemented with 5% (w/v) NaCl. Growth in tissue culture in fibroblasts is
strictly intracellular. No growth on MacConkey agar.
Isolated from subcutaneous granulomata of a patient undergoing treatment for Hodgkin’s disease. Subsequently found in skin lesions
of patients undergoing immunosuppressive treatment.
Resistant to TCH (1 µg/ml), rifampin (25 μg/ml), ethambutol (2 µg/ml), isoniazid (1 µg/ml) and streptomycin (8 µg/ml).
Experimental infection: Guinea pigs do not develop any obvious pathology after intravenous, intramuscular, or subcutaneous
inoculation with dense suspensions of the organisms. Some mice die 2-4 weeks after inoculation; numerous intracellular bacilli are
found in monocytes and macrophages of kidney, liver, and spleen, but gross lesions are not formed.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Loredana Gabriela Popa, Mircea Ioan Popa 2009. Identificarea bacililor acido-rezistenti in: Tratat de microbiologie clinica, Dumitru
Buiuc, Marian Negut, ed. a III-a, Editura Medicala, 881-890, ISBN (13) 978-973-39-0593-6.
- Sompolinsky D, Lagziel A, Naveh D, Yankilevitz L. M. haemophilum sp. nov., a new pathogen of humans. International Journal of
Systematic Bacteriology 1978; 28:67-75.
- Rastogi N, Legrand E, Sola C. The mycobacteria: an introduction to nomenclature and pathogenesis. Rev Sci Tech. 2001;20(1):21‐
- Tsukamura M. Numerical identification of slowly growing mycobacteria. Microbiol Immunol. 1985;29(11):1039‐1050. doi:10.1111/j.
Positive results for arylsulfatase (14 days), neutral red test, nicotinamidase, and pyrazinamidase.
Negative results for arylsulfatase (3 days), acid phosphatase, beta-galactosidase, catalase (inactivated at 68°C), semi-quantitative
catalase, nitrate reduction, niacin production, tellurite reduction, Tween 80 hydrolysis and urea hydrolysis.
No utilization as sole carbon source of acetate, citrate, succinate, malate, pyruvate, benzoate, fumarate, glucose, fructose, sucrose
ethanol, and propanol.
(c) Costin Stoica