Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium grossiae Paniz-Mondolfi et al. 2017.
Acid-alcohol-fast rods. Gram-positive.
Colonies are domed, smooth, dark orange/yellow and grow on 5% sheep blood agar,
Middlebrook 7H10 agar and Lowenstein-Jensen agar after 5 days at 24-42 ºC,
optimum at 37 ºC (weak growth at 42 ºC). Can grow on MacConkey agar without
crystal violet, but not on 5% NaCl media.
Isolated from sputum and blood culture specimens.
Susceptible to sulfamethoxazole+trimethoprim, linezolid, clarithromycin, ciprofloxacin, imipenem, moxifloxacin, amikacin, doxycycline
and minocycline and show intermediate susceptibility to cefoxitin.
- Paniz-Mondolfi AE, Greninger AL, Ladutko L, Brown-Elliott BA, Vasireddy R, Jakubiec W, Vasireddy S, Wallace RJJ, Simmon KE,
Dunn BE, et al. Mycobacterium grossiae sp. nov., a rapidly growing, scotochromogenic species isolated from human clinical
respiratory and blood culture specimens. Int J Syst Evol Microbiol 2017; 67:4345-4351.
Positive results for iron uptake, nitrate reductase, Tween 80 hydrolysis and urease.
Can utilize as sole carbon source: D-glucose, D-mannitol, D-sorbitol, and L-rhamnose.
Negative results for arylsulfatase (3 days), arginine dihydrolase, and ornithine decarboxylase.
No utilization of citrate and inositol.
(c) Costin Stoica