Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium gastri Wayne 1962.
Acid-alcohol-fast moderately long rods which frequently show cross-barring. A weak
or intermediate type of cording may be observed.
Smooth to rough and pale buff to white colonies are formed on inspissated egg
medium. On oleic acid-albumin agar, smooth or somewhat granular colonies
are formed after incubation at 37 ºC for 7 or more days. Temperature range for growth
is 25-40 ºC. No growth on 5% (w/v) NaCl. No growth on MacConkey agar. The
colonies do not exhibit a positive iron uptake reaction.
Isolated from a specimen of human gastric lavage. Susceptible to hydroxylamine (500 µg/ml), rifampicin (25 µg/ml), ethambutol (2
µg/ml) and isoniazid (1 µg/ml). Resistant to TCH (1 µg/ml).
It is not considered to be a cause of gastric disease in humans.
Fails to produce progressive disease in guinea pigs, but is usually capable of producing local ulceration at sites with intradermal
inoculation (0.01 mg of bacilli).
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Loredana Gabriela Popa, Mircea Ioan Popa 2009. Identificarea bacililior acido-rezistenti in: Tratat de microbiologie clinica,
Dumitru Buiuc, Marian Negut, ed. a III-a, Editura Medicala, 881-890, ISBN (13) 978-973-39-0593-6.
- Wayne LG. Classification and identification of mycobacteria. III. Species within Group III. American Review of Respiratory
Diseases 1966; 93:919-928.
- Tsukamura M. Numerical identification of slowly growing mycobacteria. Microbiol Immunol. 1985;29(11):1039‐1050. doi:10.1111/j.
- Tsukamura M. Numerical Classification of Slowly Growing Mycobacteria. International Journal of Systematic Bacteriology, Oct.
1976, p. 409-420.
- Turenne C, Chedore P, Wolfe J, Jamieson F, Broukhanski G, May K, Kabani A. Mycobacterium lacus sp. nov., a novel slowly
growing, non-chromogenic clinical isolate. Int J Syst Evol Microbiol 2002; 52:2135-2140.
- Rastogi N, Legrand E, Sola C. The mycobacteria: an introduction to nomenclature and pathogenesis. Rev Sci Tech. 2001;20(1):21‐
Closely related to Mycobacterium kansasii biochemically, but is not agglutinated by Mycobacterium kansasii-typing serum.
Positive results for acid phosphatase, arylsulfatase (10 days; negative at 3 days), neutral red test, nicotinamidase, Tween 80
hydrolysis, and urea hydrolysis.
Can utilize as sole carbon source acetate, glucose, and pyruvate.
Negative results for catalase (inactivated at 68 ºC), catalase (semi-quantitative), alpha-esterase, beta-galactosidase, iron uptake,
niacin production, nitrate reduction, pyrazinamidase.
No utilization as sole carbon source of citrate, succinate, malate, benzoate, fumarate, fructose, sucrose ethanol, and propanol.
Variable results for tellurite reduction.
(c) Costin Stoica