Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium florentinum Tortoli et al. 2005.
Member of the Mycobacterium simiae complex.
Acid-fast rods. Non-motile. Non-capsulated. Non-spore-forming. Gram-positive.
Colonies on Lowenstein-Jensen medium are smooth, creamy, and non-chromogenic
and appear after 2 weeks incubation. Grows at 25-37 ºC, but not at 42 or 45 ºC. Does
not grow on MacConkey agar or on media supplemented with 5% (w/v) NaCl.
Isolated from human clinical samples (seven from sputum, and one from lymphnode).
Resistant to isoniazid (1 μg/ml), hydroxylamine, oleic acid, p-nitrobenzoic acid, thiacetazone and ciprofloxacin. Variable results for
amikacin, ethambutol, and rifampicin.
Isolated from a patient with pulmonary infection.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tortoli E, Rindi L, Goh KS, Katila ML, Mariottini A, Mattei R, Mazzarelli G, Suomalainen S, Torkko P, Rastogi N. Mycobacterium
florentinum sp. nov., isolated from humans. Int J Syst Evol Microbiol 2005; 55:1101-1106.
Positive results for tellurite reduction (9 days) and urea hydrolysis.
Negative results for arylsulphatase (3, 7 and 10 days), niacin accumulation, and Tween 80 hydrolysis.
Variable results for catalase, semiquantitative catalase test, and nitrate reduction.
(c) Costin Stoica