Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium diernhoferi (ex Bonicke and Juhasz 1965) Tsukamura et al. 1983.
Acid-fast rods, 0.5 x 2-6 μm long. No cross-barring. No cords formation.
Forms white, smooth colonies. Non-photochromogenic. Grows within 3 days. Grows
at 28 and 37”C, but does not grow at 42, 45 or 52 ºC. No growth on MacConkey agar
w/o crystal violet. Can grow on media supplemented with 5% NaCl.
Isolated from soil in a cattle field. Resistant to isoniazid (10 μg/ml), thiophene-2-carboxylic acid hydrazide (1 μg/ml), rifampin (25
μg/ml), and p-nitrobenzoic acid (0.5 mg/ml). Susceptible to ethambutol (5 μglml) and hydroxylamine (500 μglml). Tolerant of 0.2%
picric acid and 0.1% NaNO2 in Sauton agar medium.
Considered to be non-pathogenic.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tsukamura M, Van Der Meulen HJ, Grabow WOK. Numerical taxonomy of rapidly growing, scotochromogenic mycobacteria of the
Mycobacterium parafortuitum complex: Mycobacterium austroafricanum sp. nov. and Mycobacterium diernhoferi sp. nov., nom.
rev. Int. J. Syst. Bacteriol. 1983; 33:460-469.
- Schroder KH, Naumann L, Kroppenstedt RM, Reischl U. Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic
mycobacterium. Int J Syst Bacteriol 1997; 47:86-91.
Positive results for acetamidase, beta-esterase, iron uptake, nitrate reduction, nicotinamidase, pyrazinamidase, and urease.
Acid is produced from glucose and mannose.
Can utilize as sole carbon source acetate, mannose, arabinose, xylose, inositol, mannitol, pyruvate, fructose, glucose, ethanol, and
Negative results for arylsulfatase (3 days), semiquantitative catalase test, thermo-stable catalase (68 ºC), alpha-esterase,
beta-galactosidase, niacin accumulation, Tween 80 hydrolysis, benzamidase, isonicotinamidase, and succinamidase.
No utilization as sole carbon source of acetamide, benzoate, malate, malonate, fumarate, sucrose, galactose, rhamnose, sorbitol,
Variable results for arylsulfatase (14 days), acid phosphatase, allantoinase, utilization of citrate and succinate.
(c) Costin Stoica