Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium cookii Kazda et al. 1990.
Acid-alcohol-fast, polymorphic rods (0.8 x 1.4-1.9 μm), often forming clumps. Not
forming cords. Cross banding not evident. Not producing aerial hyphae, capsules,
spores, or true branching.
Colonies on Lowenstein-Jensen medium and Middlebrook 7H10 agar are 0.5-1.0
mm in diameter and smooth, glistening with yellow-orange scotochromogenic
pigmentation. Visible growth from dilute inocula occurs after incubation for 4 weeks.
Optimal temperature for growth is 31 ºC; many strains grow at 22 ºC, but growth does
not occur at or above 37 ºC.
Isolated from sphagnum vegetation and pond water collected from sites throughout the North and South Islands of New Zealand.
Susceptible to ethambutol (2 μg/ml), isoniazid (1 μg/ml), rifampin (32 μg/ml), streptomycin (8 μg/ml), and ethionamide (40 μg/ml).
Not pathogenic for guinea pigs, mice, or rabbits, but can stimulate non-specific tuberculin reactions in sensitized guinea pigs.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Kazda J, Stackebrandt E, Smida J, Minnikin DE, Daffe M, Parlett JH, Pitulle C. Mycobacterium cookii sp. nov. Int J Syst Bacteriol
- Reischl U, Emler S, Horak Z, Kaustova J, Kroppenstedt RM, Lehn N, Naumann L. Mycobacterium bohemicum sp. nov., a new
slow-growing scotochromogenic mycobacterium. Int J Syst Bacteriol 1998; 48:1349-1355.
Positive results for acid phosphatase, arylsulphatase (3, 7 and 10 days), catalase, and semiquantitative catalase.
Negative results for nitrate reduction, acetamidase, allantoinase, benzamidase, isonicotinamidase, nicotinamidase, pyrazinamidase,
salicylamidase, succinamidase, malonamidase, Tween 80 hydrolysis, and urease
Does not grow on media containing single carbon sources or single sources of carbon plus nitrogen (glucose, D-mannose, sucrose,
sodium succinate, sodium malonate, sodium fumarate).
(c) Costin Stoica