Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium confluentis Kirschner et al. 1992.
Species description is based on a single isolated strain.
Acid-alcohol-fast coccobacilli (0.5-0.8 by 0.7-1.7 μm). Does not form spores, capsules,
or aerial hyphae.
Colonies on Lowenstein–Jensen medium after 2-4 days incubation are colorless,
smooth, and 3-5 mm in diameter. A dark pigmentation, not dependent on light, may
be observed after prolonged culture (8-12 weeks). Growth occurs at 22-41 ºC;
optimum growth occurs at 31-37 ºC. Do not grow at 42 or 45 ºC. No growth in 5% (w/v)
Isolated from a sputum specimen obtained from a healthy male.
Susceptible to isoniazid (0.25 μg/ml), streptomycin (4.0 μg/ml), and ethambutol (1.0 μg/ml).
- Kirschner P, Teske A, Schroder KH, Kroppenstedt RM, Wolters J, Bottger EC. Mycobacterium confluentis sp. nov. Int J Syst
Bacteriol 1992; 42:257-262.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Enrico Tortoli. Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s. Clinical Microbiology
Reviews, Apr. 2003, p. 319-354.
Positive results for acid phosphatase, alpha-esterase (weak), acetamidase, catalase, semiquantitative catalase (>45 mm),
thermostable catalase (68 ºC), nitrate reductase, nicotinamidase, pyrazinamidase, and urease.
Negative results for arylsulfatase (3 and 10 days), beta-esterase, benzamidase, iron uptake, isonicotinamidase, succinidamidase,
niacin accumulation, and Tween hydrolysis.
(c) Costin Stoica