Differential
characters of MAC
type strains
Acid
phosphatase
Pyrazinamidase
Acetoin
production
Esterase
C4
Tween 80
hydrolysis
Esculin
hydrolysis
Citrate
utilization
Growth
at 45ºC
Valine / Cystine
arylamidase
M. avium subsp. avium
-
+
+
-
-
-
-
+
+
M. avium subsp.
paratuberculosis
-
-
+
-
-
-
-
-
+
M. avium subsp.
silvaticum
-
-
+
-
-
-
-
+
+
M. intracellulare subsp.
intracellulare
-
+
-
+
-
-
-
+
+
M. intracellulare subsp.
chimaera
-
+
+
-
-
+
-
-
+
M. colombiense
-
+
+
-
-
+
-
-
-
M. marseillense
+
+
+
+
v
v
-
+
+
M. timonense
v
+
+
v
-
-
-
+
+
M. bouchedurhonense *
+
+
+
-
-
v
+
+
+
Legend: +,90% or more of strains are positive; − 90% or more of strains are negative; v variable/weak, * species included in M. avium
Mycobacterium colombiense
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium colombiense Murcia et al. 2006.

Member of the the Mycobacterium avium complex (MAC).
Acid-fast rods,1.39 / 0.44 μm.
Colonies appears in 3 weeks on Lowenstein-Jensen medium and on Ogawa Kudoh
and Sauton agars. Colonies are rough and non-pigmented but may develop yellowish
pigmentation with age. Temperature range for growth is 20-37 ºC. No growth in media
supplemented with 5% NaCl or on MacConkey agar.
Isolated from the blood of HIV-positive patients in Colombia, renal transplant, and from a child with cervical lymphadenopathy.
May produce pulmonary infections and lymphadenitis.
  1. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
  2. Murcia MI, Tortoli E, Menendez MC, Palenque E, Garcia MJ. Mycobacterium colombiense sp. nov., a novel member of the
    Mycobacterium avium complex and description of MAC-X as a new ITS genetic variant. Int J Syst Evol Microbiol 2006; 56:2049-
    2054.
  3. Katariina Vuorenmaa, Iskandar Ben Salah, Vincent Barlogis, Herve Chambost, Michel Drancourt. Mycobacterium colombiense
    and Pseudotuberculous Lymphadenopathy. Emerg Infect Dis. 2009 Apr; 15(4): 619–620. doi: 10.3201/eid1504.081436.
  4. Jasmine Gosal  B. Craig Lee. A case report of fatal disseminated Mycobacterium colombiense infection in a renal transplant
    recipient. Transplant Infectious Disease Volume 20, Issue 4 August 2018, e12890.
  5. Stephen Berger 2019. GIDEON Guide to Medically Important Bacteria, eBook.
  6. I. B. Salah, C. Cayrou, D. Raoult, and M. Drancourt, Mycobacterium marseillense sp. nov., Mycobacterium timonense sp. nov. and
    Mycobacterium bouchedurhonense sp. nov.,members of the Mycobacterium avium complex. International Journal of Systematic
    and Evolutionary Microbiology, vol. 59, no. 11, pp. 2803-2808, 2009.
  7. I. B. Salah, C. Cayrou, D. Raoult, and M. Drancourt, Mycobacterium marseillense sp. nov., Mycobacterium timonense sp. nov. and
    Mycobacterium bouchedurhonense sp. nov.,members of the Mycobacterium avium complex. International Journal of Systematic
    and Evolutionary Microbiology, vol. 59, no. 11, pp. 2803-2808, 2009.
Can be differentiated from the other species within the Mycobacterium avium complex by the ability to produce urease.

Positive results for acetoin production, catalase inactivated at 68 ºC, esculin hydrolysis,  iron uptake, pyrazinamidase, pyrolidonyl
arylamidase, tellurite reduction (weak reaction), and urea hydrolysis.

Negative results for acid phosphatase, niacin accumulation, and Tween hydrolysis.
No utilization of citrate as sole carbon source.

Variable results for arylsulfatase (3, 7 and 14 days), and nitrate reduction.
(c) Costin Stoica
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