Mycobacterium chlorophenolicum
Cultural characteristics
Biochemical characters
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium chlorophenolicum (Apajalahti et al. 1986) Haggblom et al. 1994.

Old synonym:
Rhodococcus chlorophenolicus Apajalahti et al. 1986.
Acid-fast rods in young cultures (fragmenting into coccoid forms in old cultures). A
thick capsule-like material surrounds the cells after 2 weeks of incubation.
Colonies are slightly mucoid, yellow to orange, scotochromogenic, after 4-8 days
incubation at 28 ºC. Good growth occurs at 18-37 ºC. No growth at 10 or 42 ºC. Growth
occurs in the presence of 0.003 and 3.0% NaCl, but not in the presence of 7% NaCl.
Isolated from chlorophenol-contaminated soil and sludge collected from different geographic locations in Finland.
Pentachlorophenol and other polychlorinated phenolic compounds are degraded.
  1. Apajalahti JHA, Kärpänoja P, Salkinoja-Salonen MS. Rhodococcus chlorophenolicus sp. nov., a chlorophenol-mineralizing
    actinomycete. Int. J. Syst. Bacteriol. 1986; 36:246-251.
  2. Haggblom MM, Nohynek LJ, Palleroni NJ, Kronqvist K, Nurmiaho-Lassila EL, Salkinoja-Salonen MS, Klatte S, Kroppenstedt RM,
    Hagglblom MM. Transfer of polychlorophenol-degrading Rhodococcus chlorophenolicus (Apajalahti et al. 1986) to the genus
    Mycobacterium as Mycobacterium chlorophenolicum comb. nov. Int J Syst Bacteriol 1994; 44:485-493.
  3. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
  4. Nouioui I, Brunet LR, Simpson D, Klenk HP, Goodfellow M. Description of a novel species of fast growing mycobacterium:
    Mycobacterium kyogaense sp. nov., a scotochromogenic strain received as Mycobacterium vaccae. Int J Syst Evol Microbiol 2018;
Positive results for acid phosphatase (negative in API ZYM in Nouioui's study), arylsulfatase (3 and 14 days), catalase, thermo-stable
catalase and Tween 80 hydrolysis.
Can utilize erythritol, L-arabinose, D- and L-arabitol, ribose (weak), D-xylose,  fructose, glucose (weak), gluconate,  inositol, mannose,
N-acetylglucosamine (weak), arbutin (weak), sucrose, trehalose, xylitol, threonine, leucine, isoleucine, valine, ethanol, n-propanol,
butanol, isobutanol m-inositol, propylene glycol, 2,3-butylene glycol, lysine, proline, gamma-aminovalerate, ethanolamine, putrescine,
spermine, betaine, acetamide and hexadecane.

Negative results for beta-galactosidase, nitrate reductase, tellurite reduction and urease.
No utilization of glycerol, amygdalin, esculin, beta-methyl-D-xyloside, sorbose, dulcitol, alpha-methyl-D-mannoside,
alpha-methyl-D-glucoside, maltose, lactose, melibiose, inulin, melezitose, raffinose, starch, glycogen, gentiobiose, D-turanose,
D-lyxose, D-tagatose, D-fucose, L-fucose, L-xylose, D-arabinose, maltose, lactose, alpha-methyl-D-glucoside, starch, 2- or
5-ketogluconate, glucuronate, saccharate, mucate, valerate, caproate, heptanoate, caprylate, caprate, malonate, maleate, adipate,
pimelate, D- and L-tartrate, glycolate, glycerate, citrate, aconitate, itaconate, geraniol, D-mandelate, L-mandelate, benzoate,
2-hydroxybenzoate, 3-hydroxybenzoate, 2-hydroxybenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-aminobenzoate, phthalate,
naphthalene, o-toluate, m-toluate, p-toluate, vanillate, isovanillate, m-anisate, p-anisate, phenylacetate, o-hydrowhenylacetate,
3,4-dimethoxybenzoate, p-hydroxyphenylacetate, p-cresol, toluene, p-alanine, methionine, glutamine, tyrosine, phenylalanine,
tryptophan, alpha-aminobutyrate, gamma-aminocaproate, alpha-amylamine, benzylamine, histamine, tryptamine, creatine,
pantothenate, trigonelline, nicotinate, and phenol (0.1 %) .

Variable utilization of D-ribose, L-rhamnose, galactose, cellobiose, sucrose, salicin, isopropanol, mannitol, sorbitol, adonitol,
ethylene glycol, phenol (0.05%), protocatechuate, quinate, glycine, L- alanine, serine aspartate, glutamate, arginine, histidine,
norleucine, alpha-aminovalerate, butylamine, sarcosine, and hippurate.
(c) Costin Stoica
Culture media
Biochemical tests
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