Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium chitae Tsukamura 1967.
Acid-fast coccoid cells. Acid-fastness may become partial in older cultures. No
branching, cord, or mycelium formation.
Colonies after 3-5 days incubation on on egg media and on Sauton agar, are white or
cream colored, smooth, wet-looking, non-pigmented. The temperature range for
growth is 28-37 ºC; does not grow at 42, 45 or 52 ºC. Grows on media containing 5%
(w/v) NaCl. Does not grow on MacConkey agar without crystal violet.
Originally isolated from soil samples collected from manure heaps.
Susceptible to hydroxylamine (500 µg/ml). Resistant to ethambutol (5 µg/ml), rifampin (25 µg/ml), and thiophen-2-carbonic acid
hydrazide (10 µg/ml).
Experimental infection: avirulent for chickens, guinea pigs, mice, and rabbits injected with 2-10 mg of the culture.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tsukamura M. Mycobacterium chitae: a new species. Jpn J Microbiol 1967; 11:43-47.
- Tsukamura M. Numerical analysis of rapidly growing, nonphotochromogenic mycobacteria, including Mycobacterium agri
(Tsukamura 1972) Tsukamura sp. nov., nom. rev. Int. J. Syst. Bacteriol. 1981; 31:247-258.
- Tsukamura M, Mizuno S, Toyama H. Mycobacterium pulveris sp. nov., a nonphotochromogenic mycobacterium with an
intermediate growth rate. Int. J. Syst. Bacteriol. 1983; 33:811-815.
Positive results for arylsulfatase (7 days), acid phosphatase, thermotolerant catalase (68 ºC), alpha- and beta-esterase, nitrate
reduction, nicotinamidase, pyrazinamidase, and urease.
Can utilize acetamide, acetate and pyruvate as sole carbon source.
Negative results for arylsulfatase (3 days), beta-galactosidase, niacin accumulation, salicylate degradation, and Tween 80 hydrolysis.
No utilization of citrate, succinate, malate, benzoate, malonate, fumarate, myo-inositol, mannitol, sorbitol, trehalose, and xylose.
Variable results for iron uptake.
(c) Costin Stoica