Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium botniense Torkko et al. 2000.
Colonies on egg media and on Middlebrook 7H11 agar are small, dysgonic and
scotochromogenic, and produce yellow pigment. Visible growth from diluted inocula
requires 5-8 weeks. Growth occurs at 37-50 ºC. Does not grow at 30 ºC and dysgonic
growth at 50 ºC. No growth in media supplemented with 5% (w/v) NaCl.
Isolated from stream water that had a high organic matter content and low pH and drained a peat land area.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Torkko P, Suomalainen S, Iivanainen E, Suutari M, Tortoli E, Paulin L, Katila ML. Mycobacterium xenopi and related organisms
isolated from stream waters in Finland and description of Mycobacterium botniense sp. nov. Int J Syst Evol Microbiol 2000; 50:283-
Positive results for arylsulfatase (10 days) and pyrazinamidase.
Negative results for arylsulfatase (3 days), nitrate reductase, semi-quantitative catalase, heat-stable catalase (68 ºC), acid
phosphatase, beta-galactosidase, Tween 80 hydrolysis (10 days) and urease.
(c) Costin Stoica