Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium bohemicum Reischl, Emler, Horak, Kaustova, Kroppenstedt, Lehn and Naumann 1998.
Acid-fast, rod-shaped cells.
Colonies on Lowenstein-Jensen medium and Middlebrook 12B agar are smooth, 1-2
mm in diameter, after 4-6 weeks incubation. Scotochromogenic. Does not grow on
media containing 5% (w/v) NaCl. Temperature range for growth is 25-40 ºC; optimal
growth temperature is 37 ºC; does not grow at 42 or 45 ºC.
Originally isolated from three consecutive sputum samples from a patient with Down’s syndrome suffering from tuberculosis.
Subsequently, the species has been isolated from children, from veterinary and from environmental sources.
Susceptibile to cycloserine (16 µg/ml), prothionamide (32 µg/ml), clarithromycin (2 µg/ml), gentamicin (2 µg/ml) and amikacin (2 µg/ml).
Resistant to isoniazid (1 µg/ml), rifampin (32 µg/ml), streptomycin (8 µg/ml), ethambutol (2 µg/ml) and ciprofloxacin (4 µg/ml).
May affect immunocompetent patients.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Reischl U, Emler S, Horak Z, Kaustova J, Kroppenstedt RM, Lehn N, Naumann L. Mycobacterium bohemicum sp. nov., a new
slow-growing scotochromogenic mycobacterium. Int J Syst Bacteriol 1998; 48:1349-1355.
- Tortoli E, Piersimoni C, Kroppenstedt RM, Montoya-Burgos JI, Reischl U, Giacometti A, Emler S. Mycobacterium doricum sp. nov.
Int J Syst Evol Microbiol 2001; 51:2007-2012.
Positive results for catalase (inactivated at 68 ºC) and urea hydrolysis (weak reaction).
Negative results for arylsulfatase (3, 7 and 10 days), acid phosphatase, catalase (semi-quantitative), alpha- and beta-esterase,
beta-galactosidase, nitrate reduction, niacin production, tellurite reduction, Tween 80 hydrolysis, benzamidase, acetamidase,
nicotinamidase, succinamidase and allantoinamidase.
Variable results for pyrazinamidase.
(c) Costin Stoica