Mycobacterium bohemicum
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium,
Mycobacterium bohemicum Reischl, Emler, Horak, Kaustova, Kroppenstedt, Lehn and Naumann 1998.
Acid-fast, rod-shaped cells.
Colonies on Lowenstein-Jensen medium and Middlebrook 12B agar are smooth, 1-2
mm in diameter, after 4-6 weeks incubation. Scotochromogenic. Does not grow on
media containing 5% (w/v) NaCl. Temperature range for growth is 25-40 ºC; optimal
growth temperature is 37 ºC; does not grow at 42 or 45 ºC.
Originally isolated from three consecutive sputum samples from a patient with Down’s syndrome suffering from tuberculosis.
Subsequently, the species has been isolated from children, from veterinary and from environmental sources.
Susceptibile to cycloserine (16 µg/ml), prothionamide (32 µg/ml), clarithromycin (2 µg/ml), gentamicin (2 µg/ml) and amikacin (2 µg/ml).
Resistant to isoniazid (1 µg/ml), rifampin (32 µg/ml), streptomycin (8 µg/ml), ethambutol (2 µg/ml) and ciprofloxacin (4 µg/ml).
May affect immunocompetent patients.
  1. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
  2. Reischl U, Emler S, Horak Z, Kaustova J, Kroppenstedt RM, Lehn N, Naumann L. Mycobacterium bohemicum sp. nov., a new
    slow-growing scotochromogenic mycobacterium. Int J Syst Bacteriol 1998; 48:1349-1355.
  3. Tortoli E, Piersimoni C, Kroppenstedt RM, Montoya-Burgos JI, Reischl U, Giacometti A, Emler S. Mycobacterium doricum sp. nov.
    Int J Syst Evol Microbiol 2001; 51:2007-2012.
Positive results for catalase (inactivated at 68 ºC) and urea hydrolysis (weak reaction).

Negative results for arylsulfatase (3, 7 and 10 days), acid phosphatase, catalase (semi-quantitative), alpha- and beta-esterase,
beta-galactosidase, nitrate reduction, niacin production, tellurite reduction, Tween 80 hydrolysis, benzamidase, acetamidase,
nicotinamidase, succinamidase and allantoinamidase.

Variable results for pyrazinamidase.
(c) Costin Stoica
Antibiogram
Encyclopedia
Culture media
Biochemical tests
Stainings
Images
Movies
Articles
Identification
Software
R E G N U M
PROKARYOTAE
Previous page
Back