Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium barrassiae Adekambi et al. 2006.
Acid-fast rods. Gram-positive.
Colonies are nonpigmented and appear on 5% sheep blood agar, Middelbrook 7H10
agar, and egg-based Lowenstein-Jensen slants in 3 to 6 days at temperatures
between 25 and 42 ºC. Can grow in 5% NaCl media.
Isolated from a bronchial aspirate.
Susceptible in vitro to imipenem, minocycline, doxycycline, clarithromycin, azithromycin, amikacin, ciprofloxacin, ofloxacin, and
sparfloxacin. Resistant to penicillin, amoxicillin, and erythromycin.
Associated with chronic pneumonia.
- Adekambi T, Raoult D, Drancourt M. Mycobacterium barrassiae sp. nov., a Mycobacterium moriokaense group species
associated with chronic pneumonia. J Clin Microbiol 2006; 44:3493-3498.
Positive results for acetoin production, beta-lactamase, nitrate reduction, iron uptake, pyrazinamidase, and urea hydrolysis.
Can utilize as sole carbon source inositol, sorbitol, and L-arabinose.
Negative results for arylsulfatase after 3 days, alkaline phosphatase, beta-galactosidase, and alpha-glucosidase.
No utilization as sole carbon source of citrate and L-rhamnose.
Variable results for pyrrolidonylarylamidase.
(c) Costin Stoica