Mycobacterium asiaticum
Cultural characteristics
Biochemical characters
Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium asiaticum Weiszfeiler et al. 1971.
Acid-fast, coccoid rods. No cording. No cross-barring.
Colonies are dysgonic, smooth and yellow-orange after incubation for 15-21 days at
37 ºC. Can grow at 42 ºC, but not at 45 ºC. Strains are usually photochromogenic, but
occasionally fail to develop pigment after exposure to light (14%); pigment is not
produced in the dark. No growth at 22 ºC, weak growth at 25 ºC. No growth on media
supplemented with 5% (w/v) NaCl or on MacConkey agar without crystal violet.
Original strains were isolated from monkeys, but few strains were isolated human sputum specimens and bronchial washings.
Resistant to rifampin (25 μg/ml), hydroxylamine (500 µg/ml), ethambutol (5 µg/ml) and tiophene-2-carboxylic acid hydrazide (1 µg/ml).
May produce human pulmonary disease. It is often a colonizer or contaminant, but can be responsible for pulmonary disease
including noncavitary disease. It can also be responsible for childhood lymphadenitis and soft tissue infections.
Experimental infection: produces focal lung lesions after intravenous inoculation of mice; may kill mice within 30-60 days.
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    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
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    Buiuc, Marian Negut, ed. a III-a, Editura Medicala, 881-890, ISBN (13) 978-973-39-0593-6.
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    Australia. Journal of Clinical Microbiology, Jan. 2010, p. 162-167, doi:10.1128/JCM.01602-09.
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    Bacteriol 1993; 43:204-209.
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    and Mycobacterium kansasii. J Clin Microbiol. 2000;38(4):1426‐1429.
  8. Kim BJ, Hong SH, Kook YH, Kim BJ. Mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely
    related to Mycobacterium gordonae. Int J Syst Evol Microbiol 2014; 64:39-45.
Positive results for acid phosphatase, alpha- and beta-esterase, arylsulfatase (10 days; negative at 3 days), catalase (inactivated at
68°C), semi-quantitative catalase test, and Tween 80 hydrolysis. Can utilize as sole carbon source glucose and pyruvate.

Negative results for beta-galactosidase, nitrate reduction, niacin production, nicotinamidase, and urea hydrolysis.
No utilization as sole carbon source of acetate, citrate, succinate, malate, benzoate, glucose, fructose, sucrose ethanol, and propanol.

Variable results for pyrazinamidase and tellurite reduction.
(c) Costin Stoica
Culture media
Biochemical tests
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