Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium aquiterrae Lee and Whang 2017.
Acid-fast rods, 0.4-0.5 x 1.0-1.2 µm. Gram-positive. Non-motile. Non-spore-forming.
Colonies are circular, convex, entire, light-yellow in colour and 0.1-0.5 mm in diameter
on R2A agar after 3 days of incubation at 25 ºC. Grows at 10-37 ºC (optimum 25 ºC),
pH 4.0-9.0 (optimum pH 7.0) and with 0-5 % (w/v) NaCl (optimum 2 % (w/v) NaCl).
Growth also occurs on Nutrient agar, Luria-Bertani agar, Tryptic soy agar, ISP2, ISP4,
PDA, Bennet’s agar, Mueller-Hinton agar and Middlebrook 7H10.
Isolated from groundwater at Daejeon in Korea.
- Lee JC, Whang KS. Mycobacterium aquiterrae sp. nov., a rapidly growing bacterium isolated from groundwater. Int J Syst Evol
Microbiol 2017; 67:4104-4110.
Positive results for acid phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, cystine arylamidase, trypsin,
naphthol-AS-BI-phosphohydrolase, alpha-galactosidase and N-acetyl-beta-glucosamininase, esculin hydrolysis, starch hydrolysis,
nitrate reduction, oxidase, acid production from erythritol, D-ribose, D-glucose, D-fructose, N-acetylglucosamine, amygdalin, arbutin,
aesculin, salicin, cellobiose, maltose, lactose, sucrose, trehalose, starch, glycogen, gentiobiose, and turanose.
Can utilize glycerol, D-fructose, D-mannose, L-rhamnose, dulicitol, inositol, D-mannitol, D-ribose, D-sorbitol, arbutin, aesculin,
salicin, cellobiose, melibiose, sucrose, trehalose, raffinose, starch, glycogen, turanose, D-fucose and gluconate.
Negative results for alkaline phosphatase, catalase, alpha-chymotrypsin, DN-ase, alpha-fucosidase, gelatin hydrolysis,
beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, lipase (C14), alpha-mannosidase, urea hydrolysis, valine
arylamidase, xanthine hydrolysis, hypoxanthine hydrolysis, L-tyrosine hydrolysis, acid production from D- and L-arabitol, glycerol, D-
and L-arabinose, D-adonitol, D- and L-fucose, gluconate, 2- and 5-ketogluconate, D-galactose, D-mannose, L-sorbose, L-rhamnose,
dulicitol, inositol, inulin, D-lyxose, D-mannitol, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, melibiose,
melezitose, raffinose, D-sorbitol, D-tagatose, xylitol, methyl beta-D-xylopyranoside, D- and L-xylose.
No utilization of erythritol, D- and L-arabinose, D-adonitol, D- and L-arabitol, methyl beta-D-xylopyranoside, D-galactose, D-glucose,
L-sorbose, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, N-acetylglucosamine, amygdalin, maltose, lactose,
inulin, melezitose, xylitol, gentiobiose, D-lyxose, D-tagatose, L-fucose, 2- and 5-ketogluconate, citrate, succinate, D- and L-xylose.
(c) Costin Stoica