Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium aichiense (ex Tsukamura 1973) Tsukamura 1981.
Acid-fast short rods, below 2 μm. Prolonged culture may lose some acid-fastness.
Mycelium is not formed. No cord formation.
Dilute inocula on egg and agar media yield yellow-orange, smooth colonies after
incubation for 3-4 days or less. Grows at 25-37 ºC, but not at 45 or 52 ºC.
Isolated from from human sputum and from soil.
Susceptible to hydroxylamine (500 μg/ml) and ethambutol (5 μg/ml). Resistant to thiophene-2-carboxylic acid hydrazide (1 μg/ml), and
rifampin (25 μg/ml).
Considered to be non-pathogenic.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tsukamura M, Mizuno S, Tsukamura S. Numerical analysis of rapidly growing, scotochromogenic mycobacteria, including
Mycobacterium obuense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov.,
nom. rev., Mycobacterium chubuense sp. nov., nom. rev., and Mycobacterium tokaiense sp. nov., nom. rev. Int. J. Syst. Bacteriol.
Positive results for acid phosphatase, arylsulfatase (3 and 7 days), beta-galactosidase, and urease.
Can utilize as sole carbon source acetate, succinate, malate, fumarate, fructose, glucose, pyruvate, and ethanol.
Negative results for catalase, semiquantitative catalase test, alpha-esterase, beta-esterase, niacin production, nitrate reduction,
nicotinamidase, pyrazinamidase, acetamidase, benzamidase, isonicotinamidase, alantoinase, sucinamidase, salicylamidase, and
Tween 80 hydrolysis (positive after 14 days incubation).
No utilization of acetamide, benzoate, citrate, and sucrose.
(c) Costin Stoica