Phylum Actinobacteria, Class Actinobacteria, Order Actinomycetales, Suborder Corynebacterineae, Family Mycobacteriaceae, Genus
Mycobacterium, Mycobacterium agri (ex Tsukamura 1972) Tsukamura 1981.
Acid-fast rods, 3-7 μm long. Frequently join to form long threads. No cord formation.
Mycelium is not formed.
Colonies are rough and non-pigmented, both in the dark and after exposure to light,
when grown for less than 5 days on egg media. The temperature range for growth
is 28-45 ºC. Does not grow at 52 ºC. No growth on MacConkey agar w/o crystal violet.
Isolated from an alkali-treated soil sample.
Susceptible to hydroxylamine (500 μg/ml) and ethambutol (5 μg/ml). Resistant to thiophene-2-carboxylic acid hydrazide (1 μg/ml), and
rifampin (25 μg/ml).
Considered to be non-pathogenic.
- John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
- Tsukamura M. Numerical analysis of rapidly growing, nonphotochromogenic mycobacteria, including Mycobacterium agri
(Tsukamura 1972) Tsukamura sp. nov., nom. rev. Int. J. Syst. Bacteriol. 1981; 31:247-258.
- Tsukamura M, Yano I, Imaeda T. Mycobacterium fortuitum subspecies acetamidolyticum, a new subspecies of Mycobacterium
fortuitum. Microbiol Immunol 1986; 30:97-110.
Positive results for catalase, semiquantitative catalase test, heat-stable catalase (68 ºC), alpha-esterase, beta-esterase,
beta-galactosidase (most strains), iron uptake, nitrate reduction, alantoinase, acetamidase, nicotinamidase, pyrazinamidase, Tween
80 hydrolysis (negative at 7 days), and urease.
Can utilize as sole carbon source acetate, glucose, malate, fumarate, inositol, propanol, pyruvate, succinate, and xylose.
Negative results for acid phosphatase, benzamidase, isonicotinamidase, salicylamidase, and niacin accumulation.
No utilization of acetamide, benzoate, citrate (most strains), arabinose, galactose, mannitol, rhamnose, sorbitol, and trehalose.
Variable results for arylsulfatase (3 days, positive after 14 days).
(c) Costin Stoica