Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Listeriaceae, Genus Listeria, Listeria rocourtiae Leclercq, Clermont, Bizet,
Grimont, Le Fleche-Mateos, Roche, Buchrieser, Cadet-Daniel, Le Monnier, Lecuit and Allerberger, 2010.
Gram-positive short rods, 0.4-0.5 x 1-2 µm, with rounded ends. Nonspore-forming.
Capsule is not formed. Motile when cultured at 4 to 30 ºC. At 4, 22 and 30 ºC, the
isolate swarmed through semisolid medium in a U-shaped tube and showed a
typical ‘umbrella’ motility in tubes of mannitol-mobility semi-solid agar.
Colonies appear bluish grey by normal illumination and a characteristic blue-green
sheen is produced by obliquely transmitted light. After 48 h of incubation on trypto-
casein-soy agar at 30 ºC, colonies are 0.5–1.0 mm in diameter, translucent and low
convex with a finely textured surface and entire margin. Nonhemolytic. CAMP-test
negative both with Rhococcus equi and Staphylococcus aureus. Optimum growth
temperature is 37 ºC; can grow at 4 ºC, but not at 42 ºC. Facultatively anaerobic.
Isolated from pre-cut lettuce in Salzburg, Austria.
Sensitive to carbapenems, rifampicin, macrolides, ciprofloxacin, levofloxacin, chloramphenicol, moxifloxacin and fusidic acid.
Resistant to cephalosporins, sulfamethoxazole, nalidixic acid, tetracyclines, trimethoprim, fosfomycin, kanamycin and streptomycin.
Avirulent.
- Leclercq A., Clermont D., Bizet C., Grimont P.A.D., Le Fleche-Mateos A., Roche S.M., Buchrieser C., Cadet-Daniel V., Le Monnier A.,
Lecuit M. and Allerberger F., 2010. Listeria rocourtiae sp. nov. IJSEM 60, 2210-2214.
Positive results for N-acetylglucosamine, catalase, esculin hydrolysis, nitrates reduction to nitrites, acid production from: adonitol,
amygdalin, arbutin, cellobiose, dulcitol, erythritol, fructose, galactose, beta-gentiobiose, D-glucose, glycerol, glycogen, inositol,
lactose, maltose, mannitol, D-mannose, melibiose, methyl alpha-D-glucoside, raffinose, rhamnose, ribose, salicin, sorbitol, sorbose,
soluble starch, trehalose, and D-xylose.
Negative results for cystine arylamidase, oxidase, acid production from: D- or L-arabinose, D-arabitol, D- or L-fucose, gluconate,
glucose 1-phosphate, 2- and 5-ketogluconate, inulin, D-lyxose, melezitose, methyl alpha-D-mannoside, sucrose, tagatose, turanose,
xylitol, and L-xylose.
Dextrin fermentation is variable.
(c) Costin Stoica