Listeria rocourtiae
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Listeriaceae, Genus Listeria, Listeria rocourtiae Leclercq, Clermont, Bizet,
Grimont, Le Fleche-Mateos, Roche, Buchrieser, Cadet-Daniel, Le Monnier, Lecuit and Allerberger, 2010.
Gram-positive  short rods, 0.4-0.5 x 1-2 µm, with rounded ends. Nonspore-forming.
Capsule is not formed. Motile when cultured at 4 to 30 ºC. At 4, 22 and 30 ºC, the
isolate swarmed through semisolid medium in a U-shaped tube and showed a
typical ‘umbrella’ motility in tubes of mannitol-mobility semi-solid agar.
Colonies appear bluish grey by normal illumination and a characteristic blue-green
sheen is produced by obliquely transmitted light. After 48 h of incubation on trypto-
casein-soy agar at 30 ºC, colonies are 0.5–1.0 mm in diameter, translucent and low
convex with a finely textured surface and entire margin. Nonhemolytic. CAMP-test
negative both with
Rhococcus equi and Staphylococcus aureus. Optimum growth
temperature is 37 ºC; can grow at 4 ºC, but not at 42 ºC. Facultatively anaerobic.
Isolated from pre-cut lettuce in Salzburg, Austria.
Sensitive to carbapenems, rifampicin, macrolides, ciprofloxacin, levofloxacin, chloramphenicol, moxifloxacin and fusidic acid.
Resistant to cephalosporins, sulfamethoxazole,  nalidixic acid, tetracyclines, trimethoprim, fosfomycin, kanamycin and streptomycin.
Avirulent.
  1. Leclercq A., Clermont D., Bizet C., Grimont P.A.D., Le Fleche-Mateos A., Roche S.M., Buchrieser C., Cadet-Daniel V., Le Monnier A.,
    Lecuit M. and Allerberger F., 2010. Listeria rocourtiae sp. nov. IJSEM 60, 2210-2214.
Positive results for N-acetylglucosamine, catalase, esculin hydrolysis, nitrates reduction to nitrites, acid production from: adonitol,
amygdalin, arbutin, cellobiose, dulcitol, erythritol, fructose, galactose, beta-gentiobiose, D-glucose, glycerol, glycogen, inositol,
lactose, maltose, mannitol, D-mannose, melibiose, methyl alpha-D-glucoside, raffinose, rhamnose, ribose, salicin, sorbitol, sorbose,
soluble starch, trehalose, and D-xylose.

Negative results for cystine arylamidase, oxidase, acid production from: D- or L-arabinose, D-arabitol, D- or L-fucose, gluconate,
glucose 1-phosphate, 2- and 5-ketogluconate, inulin, D-lyxose, melezitose, methyl alpha-D-mannoside, sucrose, tagatose, turanose,
xylitol, and L-xylose.

Dextrin fermentation is variable.
(c) Costin Stoica
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