| Brochothrix campestris |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Listeriaceae, Genus Brochothrix, Brochothrix campestris Talon, Grimont,
Gasser and Boeufgras, 1988.
Gasser and Boeufgras, 1988.
Gram-positive long and short rods, occuring singly or in pairs. Nonspore-forming.
Nonmotile.
Nonmotile.
Colonies are not pigmented, 0.7-1.0 mm in diameter after 2 days on APT agar.
Facultatively anaerobic. No growth 37 ºC. Grows at 4-30 ºC. Grows at/on: 0.01%
tetrazolium, 0.1% sodium nitrite, 6% NaCl medium. No growth in 0.05 % potassium
tellurite or 8% NaCl medium. Grows on STAA medium. No growth on acetate medium.
Range of pH is 5.0-9.0, optimum 7.0.
Facultatively anaerobic. No growth 37 ºC. Grows at 4-30 ºC. Grows at/on: 0.01%
tetrazolium, 0.1% sodium nitrite, 6% NaCl medium. No growth in 0.05 % potassium
tellurite or 8% NaCl medium. Grows on STAA medium. No growth on acetate medium.
Range of pH is 5.0-9.0, optimum 7.0.
Isolated from soil and grass.
Resistant to nalidixic acid & oxacillin. Sensitive to furadoine, tetracycline, gentamicin, polymyxin B, erythromycin, novobiocin, amikacin,
tobramycin, and ampicillin. Produces a bacteriocin, brochocin-C, active on a wide range of Gram-positive bacteria, including strains of
Brochothrix thermosphacta and species of Carnobacterium, Kurthia, Enterococcus, Lactobacillus, Pediococcus and Listeria.
Resistant to nalidixic acid & oxacillin. Sensitive to furadoine, tetracycline, gentamicin, polymyxin B, erythromycin, novobiocin, amikacin,
tobramycin, and ampicillin. Produces a bacteriocin, brochocin-C, active on a wide range of Gram-positive bacteria, including strains of
Brochothrix thermosphacta and species of Carnobacterium, Kurthia, Enterococcus, Lactobacillus, Pediococcus and Listeria.
There is no evidence for pathogenicity.
- Sneath P.H.A., 2009. Genus II. Brochothrix Sneath and Jones 1976. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig,
F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 257-
262. - Talon R., Grimont P.A.D., Grimont F., Gasser F. and Boeufgras J.M., 1988. Brochothrix campestris sp. nov. IJSB Vol. 38, No. 1, 99-
102.
Positive results for catalase (when cultured on a suitable medium: APT medium-Difco,
BAB No.2 Difco, at 20 ºC), esculin hydrolysis, glutamate arylamidase, glycyl-phenylalanine arylamidase, histidyl-L-phenylalanine
arylamidase, histidine arylamidase, methyl-red, nitrates reduction to nitrites, phenylalanine arylamidase, phosphatase, seryl-tyrosine
arylamidase, tryptophan arylamidase, Voges-Proskauer reaction, acid production from: N-acetylglucosamine, adonitol (weak),
amygdalin, cellobiose, dextrin, dulcitol, fructose, galactose, gentibose, glucose, glycerol, lactose, maltose, mannose, melibiose
(weak), raffinose, ribose, salicin, trehalose & xylose.
Negative results for arginine hydrolysis, cellulose hydrolysis, citrate utilization, deoxyribonuclease, gelatin hydrolysis, H2S production,
indole production, lecithinase, oxidase, ribonuclease, Tweens 20, 40 and 80 hydrolysis, urea hydrolysis, acid production from:
erythritol, methyl alpha-D-glucoside & sorbose.
Variable results for casein hydrolysis, Tween 60 hydrolysis, acid production from: arabinose, inulin, melezitose & sorbitol.
BAB No.2 Difco, at 20 ºC), esculin hydrolysis, glutamate arylamidase, glycyl-phenylalanine arylamidase, histidyl-L-phenylalanine
arylamidase, histidine arylamidase, methyl-red, nitrates reduction to nitrites, phenylalanine arylamidase, phosphatase, seryl-tyrosine
arylamidase, tryptophan arylamidase, Voges-Proskauer reaction, acid production from: N-acetylglucosamine, adonitol (weak),
amygdalin, cellobiose, dextrin, dulcitol, fructose, galactose, gentibose, glucose, glycerol, lactose, maltose, mannose, melibiose
(weak), raffinose, ribose, salicin, trehalose & xylose.
Negative results for arginine hydrolysis, cellulose hydrolysis, citrate utilization, deoxyribonuclease, gelatin hydrolysis, H2S production,
indole production, lecithinase, oxidase, ribonuclease, Tweens 20, 40 and 80 hydrolysis, urea hydrolysis, acid production from:
erythritol, methyl alpha-D-glucoside & sorbose.
Variable results for casein hydrolysis, Tween 60 hydrolysis, acid production from: arabinose, inulin, melezitose & sorbitol.
(c) Costin Stoica
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Differential characters of Brochothrix species:
Legend: + positive 90-100%, - negative 90-100%, [+] positive 75-89%, [-] negative 75-89%, d positive 25-74% of strains
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