- Zhang Z, Hou Q, Wang Y, Li W, Zhao H, Sun Z, Guo Z. Lactobacillus zhachilii sp. nov., a lactic acid bacterium isolated from Zha-
Chili. Int J Syst Evol Microbiol 2019; 69:2196-2201.
- Zheng J, Wittouck S, Salvetti E, Franz MAP et al., 2020. A taxonomic note on the genus Lactobacillus: Description of 23 novel
genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae.
Int J Syst Microbiol. 70, 2782-2858.
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus zhachilii Zhang,
Hou, Wang, Li, Zhao, Sun and Guo, 2019.
Homotypic synonym: Companilactobacillus zhachilii (Zhang et al. 2019) Zheng et al. 2020.
Gram-positive straight rods, 0.12-0.30 x 0.30-0.50 µm. Non-motile. Non-spore-forming.
Colonies are flat, pale yellow after 2 days of incubation at 37 ºC on MRS agar. Grows
at 15-40 ºC; optimum temperature is 30-37 ºC. Can grow in pH 4.0-9.0 and NaCl 0-
10%. Facultative anaerobic.
Isolated from Zha-Chili, a traditional fermented food made in China.
Description is based mostly on API 50 CHL (bioMerieux) results.
Positive results for acid phosphatase (weak reaction), cystine arylamidase (weak reaction), esterase C4 (weak reaction), alpha- and
beta-glucosidase, leucine arylamidase, N-acetyl-beta-glucosaminidase, valine arylamidase, Voges-Proskauer test, acid production
from amygdalin, arbutin, cellobiose, D-fructose, galactose, gentibiose, glucose (without gas production), lactose, maltose, mannose,
melezitose, salicin, sucrose, D-tagatose, trehalose, and N-acetylglucosamine.
Negative results for alkaline phosphatase, casein hydrolysis, catalase, esterase lipase C8, alpha-fucosidase, alpha- and beta-
galactosidase, beta-glucuronidase, gelatin hydrolysis, H2S production, indole production, lipase C14, alpha-mannosidase, nitrate
reduction, oxidase, starch hydrolysis, trypsin, alpha-chymotrypsin, tyrosine hydrolysis, Tween 80 hydrolysis, urease, acid production
from adonitol, D- and L-arabitol, D- and L-arabinose, dulcitol, erythritol, esculin, D- and L-fucose, glycogen, gluconate, 2- and 5-
ketogluconate, glycerol, inositol, inulin, D-lyxose, mannitol, melibiose, raffinose, rhamnose, ribose, sorbose, sorbitol, starch,
turanose, methyl alpha-D-mannopyranoside, methyl alpha-D-xyloside, methyl beta-D-xylopyranoside, xylitol, D- and L-xylose.
(c) Costin Stoica