1. Zheng J, Wittouck S, Salvetti E, Franz MAP et al., 2020. A taxonomic note on the genus Lactobacillus: Description of 23 novel
    genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae.
    Int J Syst Microbiol. 70, 2782-2858.
  2. McFrederick QS, Vuong HQ and Rothman JA, 2018. Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and
    Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers. Int J Syst Evol Microbiol 68, 1879-1884.
Lactobacillus timberlakei
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus timberlakei
McFrederick, Vuong and Rothman 2018.

Historical synonyms:
Apilactobacillus timberlakei (McFrederick et al. 2018) Zheng et al. 2020.
Gram-positive rods, 0.5-0.7/0.8-1.6 µm. Non-spore-forming. Non-motile.
Colonies arewhite  on MRS agar supplemented with 20% D-fructose. Facultative
anaerobe. Grows at 15-35 ºC (optimally at 3
0-35 ºC). Grows at pH 2.0-6.0.
Isolated from the gut of a sweat bee (Agapostemon sp.), USA and flowers. Resistant to ampicillin, kanamycin, vancomycin,
chloramphenicol
.
Undetermined.
Description is based mostly on API 50 CHL and API ZYM (bioMerieux) results / 48h.

Positive results for acid and alkaline phosphatase, catalase, leucine arylamidase (weak activity), acid production from D-fructose.

Negative results for esterase C4, acid production from adonitol, amygdalin, arbutin, D- and L-arabinose, D- and L-arabitol, cellobiose,
dulcitol, erythritol, esculin, D- and L-fucose, galactose, gentiobiose, gluconate, 2- and 5-ketogluconate, glycerol, glycogen, inositol,
inulin, D-lyxose, lactose, maltose, mannitol, mannose, melibiose, melezitose, N-acetylglucosamine, methyl alpha-D-
glucopyranoside, methyl alpha-D-xyloside, methylalpha-D-mannopyranoside, raffinose, rhamnose, ribose, salicin, L-sorbose,
sorbitol, starch, sucrose
, D-tagatose, trehalose, turanose,methyl beta-D-xylopyranoside, xylitol, D- and L-xylose.

Variable results for glucose fermentation without gas production.
(c) Costin Stoica
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