- Tohno M, Kitahara M, Irisawa T, Masuda T, Uegaki R, Ohkuma M and Tajima K, 2013. Lactobacillus silage sp. nov. isolated from
orchardgrass (Dactylis glomerata L.) silage. Int J Syst Evol Microbiol 63, 4613-4618.
- BacDive in 2019: bacterial phenotypic data for High-throughput biodiversity analysis. Reimer, L. C., Vetcininova, A., Sarda
Carbasse, J., Sohngen, C., Gleim, D., Ebeling, C., Overmann, J. Nucleic Acids Research; database issue 2019.
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus silagei Tohno,
Kitahara, Irisawa, Masuda, Uegaki, Ohkuma and Tajima 2013.
Species decription is based on a single isolate.
Gram-positive rods, 0.8 x 1.5-3.0 µm. Non-motile. Non-spore-forming.
Colonies on MRS agar are white, irregularly circular, convex, 1-2 mm in diameter, after
72 h at 30 ºC under anaerobic conditions. Facultative anaerobe. Grows at temperature
range 15-45 ºC (optimally at 30 ºC), pH range 3.5-7.5 and in 4.0% NaCl.
Isolated from orchardgrass (Dactylis glomerata L.) silage in Hachimantai, Iwate, Japan.
Description is based mostly on API 50 CHL (7 days), API ZYM, and API 20 Strep (bioMerieux) results.
Positive results for acid phosphatase, cystine aminopeptidase, esterase C4, esterase C8, alpha- and beta- galactosidase,
alpha-glucosidase, leucine aminopeptidase, valine aminopeptidase, acid production from L-arabinose, D-arabitol, fructose,
galactose, glucose (with gas production), gluconate acid (without gas production), N-acetylglucosamine, methyl
alpha-D-glucopyranoside, maltose, mannitol, melibiose, ribose, and D-xylose.
Negative results for alkaline phosphatase, arginine dihydrolase, catalase, esculin hydrolysis, alpha-fucosidase, beta-glucuronidase,
beta-glucosidase, N-acetyl-beta-glucosaminidase, lipase C14, alpha-mannosidase, trypsin, alpha-chymotrypsin, acid production
from adonitol, amygdalin, D-arabinose, L-arabitol, cellobiose, dulcitol, erythritol, esculin, fucose, beta-gentiobiose, glycerol, glycogen,
inositol, inulin, 2- and 5-ketogluconate, lactose, D-lyxose, mannose, melezitose, raffinose, rhamnose, salicin, sorbitol, sorbose,
starch, sucrose, tagatose, trehalose, turanose, xylitol, and L-xylose.
(c) Costin Stoica