Lactobacillus rhamnosus colonies on MRS agar
after 48h incubation at 37 ºC
Lactobacillus rhamnosus Gram-stained cells
Lactobacillus rhamnosus
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, [Group B lactobacilli
(facultatively heterofermentative), unique phylogenetic-group],
Lactobacillus rhamnosus (Hansen 1968) Collins, Phillips and Zanoni
1989.

Hystorical synonym:  
Lactobacillus casei subsp. rhamnosus Hansen 1968.
Rods, 0.8-1.0 x 2.0-4.0 µm,  often with square ends, occurring singly or in chains.
Nonmotile.
Can grow at 15 and 45 ºC; variable growth at 10 or 48 ºC.
Isolated from dairy products, sewage, humans (intestines), and clinical source.
Undetermined.
  1. Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus  Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 465-511.
  2. Collins M., Phillips B.A. and Zanoni P., 1989. Deoxyribonucleic Acid Homology Studies of Lactobacillus casei, Lactobacillus
    paracasei sp. nov., subsp. paracasei and subsp. tolerans, and Lactobacillus rhamnosus sp. nov., comb. nov. IJSB Vol. 39, No.2,
    105-108.
Facultatively heterofermentative (hexoses are fermented almost exclusively to lactic
acid by the Embden-Meyerhof pathway or, at least by some species, to lactic acid,
acetic acid, ethanol, and formic acid under glucose limitations.
Pentoses are  fermented to lactic and acetic acid via an inducible phosphoketolase).

Positive results for aesculin hydrolysis, fermentation of: N-acetylglucosamine,
amygdalin, arbutin, cellobiose, esculin, fructose, galactose, glucose (acid
production), gluconate, beta-gentiobiose, inositol (weak reaction) lactose, maltose,
mannitol, mannose, melezitose, rhamnose, ribose, salicin, sorbitol, sucrose,
tagatose, trehalose and turanose.

Negative results for arginine hydrolysis (NH
3 production), urease, fermentation of:
adonitol, D- or L-arabinose, D- or L-arabitol, erythritol, fucose, 2- and 5-ketogluconate,
inulin, melibiose, raffinose, starch, xylitol, D- or L-xylose.

Variable results for fermentation of: dulcitol, glycerol (weak reaction), D-lyxose (weak
reaction) and sorbose.

Catalase and oxidase negative (admin note).
(c) Costin Stoica
Antibiogram
Encyclopedia
Culture media
Biochemical tests
Stainings
Images
Movies
Articles
Identification
Software
R E G N U M
PROKARYOTAE
Previous page
Back