Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, [Group C lactobacilli
(obligately heterofermentative), unique phylogenetic group], Lactobacillus parakefiri corrig. Takizawa, Kojima, Tamura, Fujinaga,
Benno and Nakase 1994.
Gram-positive rods, 0.5-1.2 x 1.0-3.5 µm, occurring singly, in pairs, or occasionally in
short chains. Nonmotile.
Colonies on R-CW agar at 30 ºC after 5 days are 0.5-2.0 mm, circular to irregular, flat,
opaque, white and rough. Facultatively anaerobic. Grow 15 ºC, not at 45 ºC.
Isolated from kefir grains.
Undetermined.
- Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
Springer, 465-511.
- Takizawa S., Kojima S., Tamura S., Fujinaga S., Benno Y. and Nakase T., 1994. Lactobacillus kefirgranum sp. nov. and
Lactobacillus parakefir sp. nov., Two New Species from Kefir Grains . IJSB Vol. 44, No. 3, 435-439.
Obligately heterofermentative (produces L-lactic acid and CO2 by heterofermentation).
Milk is curdled. Positive results for arginine hydrolysis (NH3 from arginine), fermentation of: L-arabinose, galactose, glucose (with gas
production), lactose, maltose, and ribose.
Negative results for catalase, esculin hydrolysis, H2S production, oxidase, fermentation of: amygdalin, cellobiose, esculin, fructose,
gluconate (acid), mannitol, mannose, raffinose, rhamnose, salicin, sorbitol, sucrose, trehalose, and xylose.
Variable results for melezitose and melibiose fermentation.
(c) Costin Stoica