- Olofsson T.C., Alsterfjord M., Nilson B., Butler E. and Vasquez A., 2014. Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp.
nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus
helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis
mellifera. Int J Syst Evol Microbiol 64, 3109-3119.
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus mellifer
Olofsson, Alsterfjord, Nilson, Butler and Vasquez 2014.
Gram-positive rods, 0.5-0.8 / 5.0-9.0 µm , occuring singly or in pairs. Non-motile.
Colonies on supplemented MRS agar (0.1% L-cysteine and 2% fructose) are
colourless, 2-3 mm in diameter, under anaerobic conditions after 3 days at 35 ºC.
Grows at 15 and 50 ºC. Facultative anaerobe; grows well on supplemented MRS agar
under aerobic conditions. Can grow weakly in NaCl 5%. Range of pH 3.0-12.0 (in
supplemented MRS broth).
Isolated from the honey stomach of the honeybee, Apis mellifera mellifera.
Description is based mostly on API 50 CHL and API ZYM (bioMerieux) results.
Positive results for acid phosphatase, cystine arylamidase, esculin hydrolysis, esterase C4, esterase lipase C8, beta-glucosidase,
leucine arylamidase, valine arylamidase, acid production from arbutin, D-arabitol, esculin, fructose, glucose (without gas production)
and salicin (weak reaction).
Negative results for catalase, beta-galactosidase, alpha-glucosidase, N-acetyl-beta-glucosaminidase, acid production from adonitol,
amygdalin, D-arabinose, L-arabitol, cellobiose, dulcitol, erythritol, fucose, galactose, beta-gentiobiose, gluconate, 2- and
5-ketogluconate, glycerol, glycogen, N-acetylglucosamine, inositol, inulin, D-lyxose, maltose, mannose, melezitose, raffinose,
rhamnose, sorbitol, sorbose, starch, turanose and xylitol.
Variable results for acid production from L-arabinose (weak reaction), lactose (weak reaction), mannitol, melibiose, ribose, sucrose
(weak reaction), tagatose, trehalose and xylose (weak reaction).
(c) Costin Stoica