1. Olofsson T.C., Alsterfjord M., Nilson B., Butler E. and Vasquez A., 2014. Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp.
    nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus
    helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis
    mellifera. Int J Syst Evol Microbiol 64, 3109-3119.
Lactobacillus kimbladii
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus kimbladii
Olofsson, Alsterfjord, Nilson, Butler and Vasquez 2014.
Gram-positive rods, 0.5 / 3.0-7.0µm, occuring singly or in pairs. Non-motile.
Nonspore-forming.
Colonies on supplemented MRS agar (0.1% L-cysteine and 2% fructose) are white,
opaque, with a smooth to rough surface, raised, moist, 2-3 mm  in diameter, under
anaerobic conditions after 3 days at 35 ºC. Grows at 15 and 50 ºC; no growth at 5 ºC.
Facultative anaerobe; grows well on supplemented MRS agar under aerobic
conditions. Range of pH 3.0-12.0 (in supplemented MRS broth).
Isolated from the honey stomach of the honeybee, Apis mellifera mellifera.
No pathogenicity.
Description is based mostly on API 50 CHL and API ZYM (bioMerieux) results.

Positive results for acid phosphatase, cystine arylamidase (weak reaction), esculin hydrolysis, leucine arylamidase, valine
arylamidase, acid production from arbutin, esculin, fructose, glucose (without gas production), mannose, N-acetylglucosamine and
salicin.

Negative results for alkaline phosphatase, catalase, esterase C4, esterase C8, beta-galactosidase, N-acetyl-beta-glucosaminidase,
alpha- and beta-glucosidase, acid production from L- and D-arabinose, L- and D-arabitol, adonitol, amygdalin, cellobiose, dulcitol,
erythritol, fucose, gluconate, 2- and 5-ketogluconate, glycerol, glycogen, inositol, inulin, lactose, D-lyxose, mannitol, melezitose,
melibiose, raffinose, rhamnose, ribose, sorbitol, sorbose, starch, sucrose, trehalose, turanose, xylitol and xylose.

Variable results for acid production from arbutin, galactose, beta-gentiobiose (weak reaction), maltose (weak reaction) and tagatose.
(c) Costin Stoica
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