1. Tohno M, Kitahara M, Irisawa T, Masuda T, Uegaki R, Ohkuma M and Tajima K, 2013. Description of Lactobacillus iwatensis sp.
    nov. isolated from orchardgrass (Dactylis glomerata L.) silage, and Lactobacillus backii sp. nov. Int J Syst Evol Microbiol 63, 3854-
    3860.
Lactobacillus iwatensis
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus iwatensis
Tohno, Kitahara, Irisawa, Masuda, Uegaki, Ohkuma and Tajima 2013.
Gram-positive rods, 1.0 x 1.2-2.5 µm, occur singly, in pairs or in chains. Non-motile.
Non-spore-forming.
Colonies on MRS agar are yellow-white, smooth, convex, 0.3-0.5 mm in diameter,
after 72 h at 30 ºC under anaerobic conditions. Facultative anaerobe. Grows at
temperature range 4-37 ºC (optimally at 30 ºC), pH range 3.5-7.0 and in 4.0-6.5%
NaCl.
Isolated from orchardgrass (Dactylis glomerata L.) silage in Iwate, Japan
Undetermined.
Homofermentative.
Description is based mostly on API 50 CHL, API ZYM, API 20 Strep (bioMerieux) results.

Positive results for acid phosphatase, esterase C4, leucine arylamidase, valine arylamidase, acid production from fructose, glucose
(without gas production), N-acetylglucosamine, mannitol, and mannose.

Negative results for alkaline phosphatase, arginine dihydrolase, catalase, cystine arylamidase, esterase lipase, esculin hydrolysis,
alpha-fucosidase, alpha- and beta-glucosidase, alpha- and beta-galactosidase, beta-glucuronidase,
N-acetyl-beta-glucosaminidase, lipase C14, alpha-mannosidase, trypsin, alpha-chymotrypsin, acid production from adonitol,
amygdalin, L- and D-arabinose, L-and D-arabitol, arbutin, cellobiose, dulcitol, erythritol, esculin, fucose, galactose, beta-gentiobiose,
gluconate, methyl alpha-D-glucopyranoside, glycerol, glycogen, inositol, inulin, 2- and 5-ketogluconate, lactose, D-lyxose, maltose,
melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, sorbose, starch, sucrose, tagatose, trehalose, turanose, xylitol,
and xylose.
(c) Costin Stoica
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Cystine
arylamidase
Esterase
C4
Beta-
galactosidase
Beta-
glucosidase
N-acetyl-beta-
glucosaminidase
Arbutin
Salicin
Sorbitol
L. backii
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L. iwatensis
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Differential characters of L. backii and L. iwatensis