1. Tohno M, Kitahara M, Uegaki R, Irisawa T, Ohkuma M and Tajima K, 2013. Lactobacillus hokkaidonensis sp. nov., isolated from
    subarctic timothy grass(Phleum pratense L.) silage. Int J Syst Evol Microbiol 63, 2526-2531.
  2. BacDive in 2019: bacterial phenotypic data for High-throughput biodiversity analysis. Reimer, L. C., Vetcininova, A., Sarda
    Carbasse, J., Sohngen, C., Gleim, D., Ebeling, C., Overmann, J. Nucleic Acids Research; database issue 2019.
Lactobacillus hokkaidonensis
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus hokkaidonensis
Tohno, Kitahara, Uegaki, Irisawa, Ohkuma and Tajima 2013.
Gram-positive rods, 0.8-1.2 x 1.5-3.0 µm, occuring singly and in pairs. Non-motile.
Non-spore-forming.
Colonies on MRS agar are white, smooth, convex, small (0.5-1.0 mm in diameter, after
72 h at 25 ºC under anaerobic conditions. Facultative anaerobe. Grows at temperature
range 4-37 ºC (optimally at 25 ºC), pH range 4.0-7.0 and in 4.0% NaCl.
Isolated from timothy grass (Phleum pratense L.) silage in Hokkaido, Japan.
Undetermined.
Heterofermentative.
Description is based mostly on API 50 CHL, API ZYM, and API 20 Strep (bioMerieux) results.

Positive results for acid phosphatase, cystine arylamidase, esterase C4, esterase lipase, alpha- and beta-galactosidase, beta-
glucuronidase, alpha-glucosidase, leucine arylamidase, valine arylamidase, acid production from L- arabinose, glucose (with gas
production), N-acetylglucosamine, maltose, ribose, D-xylose, and methyl beta-D-xylopyranoside.

Negative results for alkaline phosphatase, arginine dihydrolase, catalase, esculin hydrolysis, lipase C14, alpha-mannosidase,
alpha-fucosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase, trypsin, alpha-chymotrypsin, acid production from adonitol,
amygdalin, arbutin, D-arabinose, L-arabitol, cellobiose, dulcitol, erythritol, esculin, fructose, fucose, galactose, beta-gentiobiose,
glycerol, glycogen, methyl alpha-D-glucopyranoside, inositol, inulin, 2- and 5-ketogluconate, lactose, mannitol, mannose, melezitose,
raffinose, rhamnose, salicin, sorbitol, sorbose, starch, sucrose, tagatose, trehalose, turanose, xylitol, and L-xylose.

Variable results for acid production from D-arabitol, gluconate, D-lyxose and melibiose.
(c) Costin Stoica
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