Amygdalin
Cellobiose
Lactose
Maltose
Mannose
Salicin
Sucrose
Trehalose
subsp. bulgaricus
-
d
+
-
-
-
-
-
subsp. delbrueckii
-
-
-
d
+
-
+
d
subsp. indicus
-
-
+
-
+
-
d
-
subsp. lactis
+
d
+
+
+
+
+
+
subsp. sunkii
+
+
-
+
+
+
+
+
Lactobacillus delbrueckii subsp. delbrueckii
Lactobacillus delbrueckii colonies on CASO agar
Gram-stained cells grouped in short chains or singly
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae,
Genus Lactobacillus [fermentation-type Group A lactobacilli (obligately
homofermentative), Lactobacillus delbrueckii phylogenetic-group],
Lactobacillus
delbrueckii subsp. delbrueckii  
(Leichmann 1896) Weiss, Schillinger and Kandler
1984. Type species of the genus.

Historical synonyms:  
Lactobacillus delbrueckii (Leichmann 1896) Beijerinck 1901,
Bacillus Delbrücki Leichmann 1896, Bacillus acidificans longissimus Lafar 1896,
Bacterium delbrücki Leichmann (1896) Migula 1900, Thermobacterium cereale
Orla-Jensen 1919, Lactobacterium delbrücki (Leichmann 1896) van Steenberge
1920,
Ulvina delbrücki (Leichmann 1896) Pribram 1933, Plocamobacterium
delbrücki
(Leichmann 1896) Pribram 1933.
Gram-positive rods with rounded ends, 0.5-0.8 / 2.0-9.0 µm, occuring singly and
in short chains; internal granulation may be revealed with methylene blue stain.
Nonmotile.
Colonies are normally rough and non-pigmented becoming smooth and compact in
the presence of Tween 80 or sodium oleate. Surface growth is greatly enhanced by
reduced O
2-tension or anaerobiosis.
Optimum temperature for growth is 40-44 ºC. Variable growth at 48-52 ºC. Can grow
at 45 ºC. No growth at 15 ºC. Optimum pH for growth 5.5-6.2. Can grow at pH 5.0.
Growth factor requirements: pantothenic acid and niacin generally essential;
riboflavin, folic acid, vitamin B
12, and thymidine are essential for particular strains.
Isolated from plant material fermented at high temperatures (40-53 ºC). It is present in intestinal tract of human and animal (pig,
mouse, rat) and vaginal tract.
Human local infections are very rare.
  1. Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 465-511.
  2. Rogosa M., 1974. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) Buchanan R.E. and Gibbons N.E., Bergey’s Manual of
    Determinative Bacteriology, Eighth Edition, The Williams & Wilkins Company, Baltimore, 576-593.
  3. Kudo Y, Oki K, Watanabe K. Lactobacillus delbrueckii subsp. sunkii subsp. nov., isolated from sunki, a traditional Japanese
    pickle. Int J Syst Evol Microbiol 2012; 62:2643-2649.
Obligately homofermentative (hexoses are fermented almost exclusively to lactic acid by
the Embden-Meyerhof pathway; pentoses or gluconate are not fermented).

Positive results for fermentation of: fructose, glucose (without gas), mannose, and
sucrose.

Negative results for esculin hydrolysis,  indole production, H
2S production, catalase,
oxidase, gelatin liquefaction, casein digestion, nitrate reduction, acid production from:
amygdalin, arabinose, cellobiose, esculin, galactose, gluconate, lactose, mannitol,
melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, and xylose.

Variable results for arginine hydrolysis, acid production from: maltose, and trehalose.
(c) Costin Stoica
Antibiogram
Encyclopedia
Culture media
Biochemical tests
Stainings
Images
Movies
Articles
Identification
Software
R E G N U M
PROKARYOTAE
Previous page
Back
Differential characters of Lactobacillus delbrueckii subspecies:
Legend: +  positive 90-100%, - negative 90-100%, [+] positive 75-89%, [-] negative 75-89%, d positive 25-74% of strains