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| Lactobacillus delbrueckii subsp. delbrueckii |
| Lactobacillus delbrueckii colonies on CASO agar |
| Gram-stained cells grouped in short chains or singly |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae,
Genus Lactobacillus [fermentation-type Group A lactobacilli (obligately
homofermentative), Lactobacillus delbrueckii phylogenetic-group], Lactobacillus
delbrueckii subsp. delbrueckii (Leichmann 1896) Weiss, Schillinger and Kandler
1984. Type species of the genus.
Historical synonyms: Lactobacillus delbrueckii (Leichmann 1896) Beijerinck 1901,
Bacillus Delbrücki Leichmann 1896, Bacillus acidificans longissimus Lafar 1896,
Bacterium delbrücki Leichmann (1896) Migula 1900, Thermobacterium cereale
Orla-Jensen 1919, Lactobacterium delbrücki (Leichmann 1896) van Steenberge
1920, Ulvina delbrücki (Leichmann 1896) Pribram 1933, Plocamobacterium
delbrücki (Leichmann 1896) Pribram 1933.
Genus Lactobacillus [fermentation-type Group A lactobacilli (obligately
homofermentative), Lactobacillus delbrueckii phylogenetic-group], Lactobacillus
delbrueckii subsp. delbrueckii (Leichmann 1896) Weiss, Schillinger and Kandler
1984. Type species of the genus.
Historical synonyms: Lactobacillus delbrueckii (Leichmann 1896) Beijerinck 1901,
Bacillus Delbrücki Leichmann 1896, Bacillus acidificans longissimus Lafar 1896,
Bacterium delbrücki Leichmann (1896) Migula 1900, Thermobacterium cereale
Orla-Jensen 1919, Lactobacterium delbrücki (Leichmann 1896) van Steenberge
1920, Ulvina delbrücki (Leichmann 1896) Pribram 1933, Plocamobacterium
delbrücki (Leichmann 1896) Pribram 1933.
Gram-positive rods with rounded ends, 0.5-0.8 / 2.0-9.0 µm, occuring singly and
in short chains; internal granulation may be revealed with methylene blue stain.
Nonmotile.
in short chains; internal granulation may be revealed with methylene blue stain.
Nonmotile.
Colonies are normally rough and non-pigmented becoming smooth and compact in
the presence of Tween 80 or sodium oleate. Surface growth is greatly enhanced by
reduced O2-tension or anaerobiosis.
Optimum temperature for growth is 40-44 ºC. Variable growth at 48-52 ºC. Can grow
at 45 ºC. No growth at 15 ºC. Optimum pH for growth 5.5-6.2. Can grow at pH 5.0.
Growth factor requirements: pantothenic acid and niacin generally essential;
riboflavin, folic acid, vitamin B12, and thymidine are essential for particular strains.
the presence of Tween 80 or sodium oleate. Surface growth is greatly enhanced by
reduced O2-tension or anaerobiosis.
Optimum temperature for growth is 40-44 ºC. Variable growth at 48-52 ºC. Can grow
at 45 ºC. No growth at 15 ºC. Optimum pH for growth 5.5-6.2. Can grow at pH 5.0.
Growth factor requirements: pantothenic acid and niacin generally essential;
riboflavin, folic acid, vitamin B12, and thymidine are essential for particular strains.
Isolated from plant material fermented at high temperatures (40-53 ºC). It is present in intestinal tract of human and animal (pig,
mouse, rat) and vaginal tract.
mouse, rat) and vaginal tract.
Human local infections are very rare.
- Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
Springer, 465-511. - Rogosa M., 1974. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) Buchanan R.E. and Gibbons N.E., Bergey’s Manual of
Determinative Bacteriology, Eighth Edition, The Williams & Wilkins Company, Baltimore, 576-593. - Kudo Y, Oki K, Watanabe K. Lactobacillus delbrueckii subsp. sunkii subsp. nov., isolated from sunki, a traditional Japanese
pickle. Int J Syst Evol Microbiol 2012; 62:2643-2649.
Obligately homofermentative (hexoses are fermented almost exclusively to lactic acid by
the Embden-Meyerhof pathway; pentoses or gluconate are not fermented).
Positive results for fermentation of: fructose, glucose (without gas), mannose, and
sucrose.
Negative results for esculin hydrolysis, indole production, H2S production, catalase,
oxidase, gelatin liquefaction, casein digestion, nitrate reduction, acid production from:
amygdalin, arabinose, cellobiose, esculin, galactose, gluconate, lactose, mannitol,
melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, and xylose.
Variable results for arginine hydrolysis, acid production from: maltose, and trehalose.
the Embden-Meyerhof pathway; pentoses or gluconate are not fermented).
Positive results for fermentation of: fructose, glucose (without gas), mannose, and
sucrose.
Negative results for esculin hydrolysis, indole production, H2S production, catalase,
oxidase, gelatin liquefaction, casein digestion, nitrate reduction, acid production from:
amygdalin, arabinose, cellobiose, esculin, galactose, gluconate, lactose, mannitol,
melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, and xylose.
Variable results for arginine hydrolysis, acid production from: maltose, and trehalose.
(c) Costin Stoica
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Differential characters of Lactobacillus delbrueckii subspecies:
Legend: + positive 90-100%, - negative 90-100%, [+] positive 75-89%, [-] negative 75-89%, d positive 25-74% of strains
