Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus [Group A lactobacilli (obligately
homofermentative), Lactobacillus delbrueckii phylogenetic-group], Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and
Holdeman (1970), emend. Cato, Moore and Johnson 1983.
Historical synonyms: Eubacterium crispatum Brygoo and Aladame 1953,
Lactobacillus acidophilus group A2, Johnson et al. 1980.
Gram-positive, straight to slightly curved rods with rounded ends (0.8-1.6 x 2.3-11.0
μm) occurring singly and in short chains. Nonmotile. Nonspore-forming.
After 2 days on BHI blood agar in anaerobic atmosphere (10% CO2 and 90% H2).
Colonies are minute (1.0 mm in diameter), entire to slightly scalloped, convex to
pulvinate, translucent at the edge to opaque in the center, and grayish white to tan.
Colonies have a smooth shiny surface; a granular or mosaic internal structure
occasionally is observed. Some strains can growth slightly on blood agar plates
incubated in air. In broth media containing a fermentable carbohydrate, there is
slight turbidity and a smooth to granular sediment. Growth is stimulated in the presence of 0.02% Tween 80. No growth in 6.5%
Optimum growth temperature 37 ºC. Can grow at 45 ºC, but not at 15 ºC. Weak growth at 25 ºC.
Isolated from human buccal cavities and from a patient with purulent pleurisy; strains also have been isolated from clinically healthy
vaginal tracts, from a patient with leukorrhea, from a patient with a urinary tract infection, and from the crops and ceca of chickens.
Present in intestines of pig and horse.
Susceptible to chloramphenicol, clindamycin, and erythromycin.
- Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Cato E.P., Moore W.E.C. and Johnson J.L. ,1983. Synonymy of strains of Lactobacillus acidophilus Group A2 (Johnson et 81.
1980) with the type strain of Lactobacillus crispatus (Brygoo and Aladame 1953) Moore and Holdeman 1970. IJSB vol. 33, no. 2,
Obligately homofermentative (hexoses are fermented almost exclusively to lactic acid by the Embden-Meyerhof pathway; pentoses or
gluconate are not fermented).
Positive results for esculin hydrolysis, starch hydrolysis, fermentation of: cellobiose, esculin, fructose, galactose, glucose (weak gas
production), lactose, maltose, mannose, salicin, sucrose and starch.
Negative results for arginine hydrolysis,catalase, gelatin digestion, H2S production, hippurate hydrolysis, indole, lecithinase, lipase,
milk digestion, meat digestion, nitrate reduction, urease, fermentation of: arabinose, dulcitol, inositol, mannitol, melezitose,
rhamnose, ribose, sorbitol, trehalose and xylose.
Variable results for amygdalin, erythritol, glycogen, melibiose and raffinose.
(c) Costin Stoica