- Killer J., Votavova A., Valterova I., Vlkova E., Rada V. and Hroncova Z., 2014. Lactobacillus bombi sp. nov., isolated from the
digestive tract of laboratory-reared bumblebee queens (Bombus terrestris). Int J Syst Evol Microbiol 64, 2611-2617.
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus bombi Killer,
Votavova, Valterova, Vlkova, Rada and Hroncova 2014.
Gram-positive slightly curved long rods, 0.7-1.0/2.2-7.2 µm, occur singly or in pairs.
Colonies on MRS agar are cream, disc-shaped or triangular in profile , 1.2-2.8 mm in
diameter, under anaerobic conditions after 3 days at 35 ºC. Best growth in anaerobic
TPY and MRS agar or broth, weaker growth under microaerophilic conditions on
same media. Grows at temperature range 20-47 ºC (optimally at 37 ºC), pH range
Isolated from the digestive tract of laboratory-reared bumblebee queens, Bombus terrestris.
Description is based mostly on API 50 CHL, API ZYM, Rapid ID 32A (bioMerieux) results.
Positive results for arginine arylamidase, alpha- and beta-galactosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase, leucine
arylamidase, proline arylamidase, valine arylamidase, acid production from amygdalin, arbutin, esculin, fructose, beta-gentiobiose,
glucose (acid without gas production), N-acetylglucosamine, D-lyxose, mannose, melibiose, raffinose, salicin, sucrose, and trehalose.
Negative results for catalase, cystine arylamidase, esculin hydrolysis, esterase (C8), alpha-fucosidase, gelatin hydrolysis,
alpha-glucosidase, beta-glucuronidase, indole production, lipase (C14), nitrate reduction, oxidase, trypsin, acid production from
adonitol, D-arabinose, D- and L-arabitol, dulcitol, erythritol, fucose, gluconate, glycerol, glycogen, inositol, inulin, 2- and 5-ketogluconate,
lactose, maltose, mannitol, melezitose, ribose, sorbitol, sorbose, starch, tagatose, turanose, xylitol, and xylose.
Variable results for acid and alkaline phosphatase, alanine arylamidase, tyrosine arylamidase, urease, acid production from L-
arabinose, cellobiose, galactose, and rhamnose.
(c) Costin Stoica