1. Killer J., Votavova A., Valterova I., Vlkova E., Rada V. and Hroncova Z., 2014. Lactobacillus bombi sp. nov., isolated from the
    digestive tract of laboratory-reared bumblebee queens (Bombus terrestris). Int J Syst Evol Microbiol 64, 2611-2617.
Lactobacillus bombi
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus  bombi Killer,
Votavova, Valterova, Vlkova, Rada and Hroncova 2014.
Gram-positive slightly curved long rods, 0.7-1.0/2.2-7.2 µm, occur singly or in pairs.
Non-motile. Nonspore-forming.
Colonies on MRS agar are cream, disc-shaped or triangular in profile , 1.2-2.8 mm in
diameter, under anaerobic conditions after 3 days at 35 ºC. Best growth in anaerobic
TPY and MRS agar or broth, weaker growth under microaerophilic conditions on
same media. Grows at temperature range 20-47 ºC (optimally at 37
ºC), pH range
4.0-8.5.
Isolated from the digestive tract of laboratory-reared bumblebee queens, Bombus terrestris.
No pathogenicity.
Description is based mostly on API 50 CHL, API ZYM, Rapid ID 32A (bioMerieux) results.

Positive results for arginine arylamidase, alpha- and beta-galactosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase, leucine
arylamidase, proline arylamidase, valine arylamidase, acid production from amygdalin, arbutin, esculin, fructose, beta-gentiobiose,
glucose (acid without gas production), N-acetylglucosamine, D-lyxose, mannose, melibiose, raffinose, salicin, sucrose, and trehalose.

Negative results for catalase, cystine arylamidase, esculin hydrolysis, esterase (C8), alpha-fucosidase, gelatin hydrolysis,
alpha-glucosidase, beta-glucuronidase, indole production, lipase (C14), nitrate reduction, oxidase, trypsin, acid production from
adonitol, D-arabinose, D- and L-arabitol, dulcitol, erythritol, fucose, gluconate, glycerol, glycogen, inositol, inulin, 2- and 5-ketogluconate,
lactose, maltose, mannitol, melezitose, ribose, sorbitol, sorbose, starch, tagatose, turanose, xylitol, and xylose.

Variable results for acid and alkaline phosphatase, alanine arylamidase, tyrosine arylamidase, urease, acid production from L-
arabinose, cellobiose, galactose, and rhamnose.
(c) Costin Stoica
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