Differential characters of L. backii and L. iwatensis
 
Cystine
arylamidase
Esterase C4
Beta-
galactosidase
Beta-
glucosidase
N-acetyl-beta-
glucosaminidase
Arbutin
Salicin
Sorbitol
L. backii
+
-
+
+
+
+
+
+
L. iwatensis
-
+
-
-
-
-
-
-
  1. Tohno M, Kitahara M, Irisawa T, Masuda T, Uegaki R, Ohkuma M and Tajima K, 2013. Description of Lactobacillus iwatensis sp.
    nov. isolated from orchardgrass (Dactylis glomerata L.) silage, and Lactobacillus backii sp. nov. Int J Syst Evol Microbiol 63, 3854-
    3860.
Lactobacillus backii
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus, Lactobacillus backii Tohno,
Kitahara, Irisawa, Masuda, Uegaki, Ohkuma and Tajima 2013.
Gram-positive rods, 0.7 x 3.0 µm, occur singly or in pairs. Non-spore-forming.
Non-motile.
Colonies on MRS agar are white, smooth, convex, 0.2-0.8 mm in diameter, after 72 h
at 30 ºC under anaerobic conditions. Facultative anaerobe. Grows at temperature
range 10-37 ºC (optimally at 30 ºC), pH range 3.5-8.0 and in 4.0-6.5% NaCl.
Isolated from spoiled lager beer.
Undetermined.
Homofermentative.
Description is based mostly on API 50 CHL, API ZYM, API 20 Strep (bioMerieux) results.

Positive results for acid phosphatase, cystine arylamidase, beta-galactosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase,
leucine arylamidase, valine arylamidase, acid production from arbutin, fructose, glucose (without gas production), mannitol,
mannose, sorbitol, salicin, and N-acetylglucosamine.

Negative results for alkaline phosphatase, arginine dihydrolase, catalase, esterase C4, esterase lipase, esculin hydrolysis,
alpha-fucosidase, alpha-glucosidase, alpha-galactosidase, beta-glucuronidase, lipase C14, alpha-mannosidase, trypsin,
alpha-chymotrypsin, acid production from adonitol, amygdalin, L- and D-arabinose, L- and D-arabitol, cellobiose, dulcitol, erythritol,
esculin, fucose, galactose, beta-gentiobiose, gluconate, methyl alpha-D-glucopyranoside, glycerol, glycogen, inositol, inulin, 2- and
5-ketogluconate, lactose, D-lyxose, maltose, melezitose, melibiose, raffinose, rhamnose, ribose, sorbose, starch, sucrose, tagatose,
trehalose, turanose, xylitol, and xylose.
(c) Costin Stoica
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