Phylum Firmicutes, Class Bacilli, Order Lactobacillales, Family Lactobacillaceae, Genus Lactobacillus [Group A lactobacilli (obligately
homofermentative), Lactobacillus delbrueckii phylogenetic-group], Lactobacillus amylovorus Nakamura 1981.
Synonyms: Lactobacillus acidophilus group A 3 Johnson et al. (1980); Lactobacillus
sobrius Konstantinov, Poznanski, Fuentes, Akkermans, Smidt and De Vos 2006 is a
later synonym of Lactobacillus amylovorus Nakamura 1981.
Gram-positive rods (1.0 x 3–5 μm) occurring singly and in short chains. Nonmotile.
Colonies on LBS and modified MRS agar have entire edges and are white, smooth,
convex, and opaque. Clear zones of hydrolysis surrounded colonies that grew on
LBS-corn starch agar.
Facultatively anaerobic. Optimum growth temperature 37-45 ºC. No growth at 15 ºC.
Grow at pH 4.5-8.0. Growth factor requirements: niacin, pantothenic acid, folic acid,
and riboflavin are essential.
Isolated from cattle waste-corn fermentation. Present in intestines of pig, cattle and horse.
- Hammes W.P. and Hertel C., 2009. Genus I. Lactobacillus Beijerinck 1901. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Nakamura L.K., 1981. Lactobacillus amylovorus, a new starch-hydrolyzing species from cattle waste-corn fermentations. IJSB vol.
31, No. 1, 56-63.
- Sergey R. Konstantinov, Elisa Poznanski, Susana Fuentes, Antoon D. L. Akkermans, Hauke Smidt, and Willem M. de Vos
Lactobacillus sobrius sp. nov., abundant in the intestine of weaning piglets. Int J Syst Evol Microbiol January 2006 56:29-32,
Obligately homofermentative (hexoses are fermented almost exclusively to lactic acid by the Embden-Meyerhof pathway; pentoses or
gluconate are not fermented).
Extracellular amylolytic enzyme activity.
Positive results for fermentation of: amygdalin (in 4 or more days), cellobiose, esculin (in 4 or more days), fructose, galactose,
glucose (without gas production), maltose, mannose, salicin (in 4 or more days), sucrose, starch & trehalose.
Negative results for catalase, nitrate reduction, oxidase, fermentation of: arabinose, gluconate, melezitose, melibiose, raffinose,
rhamnose, ribose, sorbitol & xylose.
Variable results for fermentation of: lactose (in 4 or more days) & mannitol (in 4 or more days).
(c) Costin Stoica