Phylum Proteobacteria, Class Epsilonproteobacteria, Order Campylobacterales, Family Helicobacteraceae, Genus Helicobacter,
Helicobacter muridarum Lee, Phillips, O’Rourke, Paster, Dewhirst, Fraser, Fox, Sly, Romaniuk, Trust and Kouprach
Gram-negative, helical rods, 3.5-5 x 0.5-0.6 μm with 2-3 spiral turns. Cells transform
to coccoid forms with age. Rapid, darting motility by means of 10-14 bipolar
sheathed flagella. Cells are surrounded by 9-11 periplasmic fibers, which appear as
concentric helical ridges on the cell surface. No endospores are produced.
Nutritionally fastidious. Grow as a fine, translucent spreading film on media enriched
with blood or serum. Nonhemolytic. Optimal growth occurs on a moist agar surface
after 2-3 days incubation at 37 ºC in a microaerobic atmosphere (1–16% oxygen, with
added 5-10% CO2). Single colonies are rarely obtained due to the preference of the
bacterium for moist agar surfaces. No growth at 25 ºC, 42 ºC, in 1.5% NaCl, 1% bile,
or 0.1% sodium fluoride.
Isolated from the intestinal mucosa of rodents (rats and mice), mainly colonizing the mucus in the ileal crypts. In germ-free animals
colonizes the cecal pits in large numbers. In older animals may colonize the gastric mucosa in large numbers.
Sensitive to cefoperazone and metronidazole. Resistant to nalidixic acid and cephalothin.
Invades small intestinal tissue in immunologically compromised mice.
- George M. Garrity, Julia A. Bell and Timothy Lilburn, 2001. Family II. Helicobacteraceae. In: Bergey’s Manual of Systematic
Bacteriology, Second edition, Vol 2, part C, George M. Garrity (Editor-in-Chief), pp 1168-1195.
- J. H. Simmons, L. K. Riley, C. L. Besch-Williford, and C. L. Franklin. Helicobacter mesocricetorum sp. nov., a Novel Helicobacter
Isolated from the Feces of Syrian Hamsters. J. Clin. Microbiol. May 2000 vol. 38 no. 5 1811-1817.
- Original Papers Relating to Systematic Bacteriology: A. Lee, M. W. Phillips, J. L. O'rourke, B. J. Paster, F. E. Dewhirst, G. J. Fraser,
J. G. Fox, L. I. Sly, P. J. Romaniuk, T. J. Trust, and S. Kouprach. Helicobacter muridarum sp. nov., a Microaerophilic Helical
Bacterium with a Novel Ultrastructure Isolated from the Intestinal Mucosa of Rodents. Int J Syst Bacteriol January 1992 42:27-36.
Positive for alkaline phosphatase, catalase, oxidase, urease & arginine aminopeptidase.
Negative results for lipase, lecithinase, indole production, H2S production, gelatin hydrolysis, casein hydrolysis, starch hydrolysis,
tributyrin hydrolysis, hippurate hydrolysis, nitrates reduction, leucine aminopeptidase, gamma-glutamyl transpeptidase, histidine
aminopeptidase, acid production from arabinose, cellobiose, galactose, glucose, glycerol, inositol, lactose, maltose, mannitol,
raffinose, ribose, salicin, sorbitol, starch, sucrose & xylose.
(c) Costin Stoica