Phylum Proteobacteria, Class Epsilonproteobacteria, Order Campylobacterales, Family Helicobacteraceae, Genus Helicobacter,
Helicobacter ganmani Robertson, O’Rourke, Vandamme, On and Lee 2001.
Curved to spiral (2 turns per cell) rods 0.3 x 2.5 μm. Motile by means of single bipolar
Culture on agar media containing 5% horse blood under anaerobic conditions
occasionally results in translucent, irregular, and unpigmented colonies 1μm in
diameter. Anaerobic; no growth in aerobic or microaerobic conditions (though there is
a report of growth both anaerobically and microaerobically). Nonhemolitic. Growth is
frequently seen as a thin spreading film. No growth at 18, 25 or 42 ºC. Optimum
growth temperature is 37 ºC. No growth in 1% glycine, 2-4% NaCl, 32 mg nalidixic
acid /L, 0.0005% crystal violet, 0.1% janus green or 0.02% pyronin. Grow on CCD
agar. Variable growth on MacConkey agar, 0.032% methyl orange media, 0.005%
basic fuchsin media, and 4 mg metronidazole /L.
Isolated from the ceca, large and small bowels, and liver of laboratory mice. Resistant to cephalothin (32 μg/l), carbenicillin (32 μg/l),
and cefoperazone (64 μg/l).
- George M. Garrity, Julia A. Bell and Timothy Lilburn, 2001. Family II. Helicobacteraceae. In: Bergey’s Manual of Systematic
Bacteriology, Second edition, Vol 2, part C, George M. Garrity (Editor-in-Chief), pp 1168-1195.
- B. R. Robertson, J. L. O'Rourke, P Vandamme, S. L. On, and A. Lee. Helicobacter ganmani sp. nov., a urease-negative anaerobe
isolated from the intestines of laboratory mice. Int J Syst Evol Microbiol September 2001 51:1881-9.
- Nancy S. Taylor, Shilu Xu, Prashant Nambiar, Floyd E. Dewhirst, and James G. Fox. Enterohepatic Helicobacter Species Are
Prevalent in Mice from Commercial and Academic Institutions in Asia, Europe, and North America. J. Clin. Microbiol. July 2007 45:
Positive results for oxidase, nitrate reduction & TTC reduction.
Negative results for alkaline phosphatase, catalase (weak reactions may occur), DN-ase, urease, hippurate hydrolysis, indoxyl
acetate hydrolysis, selenite, H2S production (TSI) & sugar fermentation in TSI.
(c) Costin Stoica