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4-aminobutyrate utilization
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Trans- aconitate utilization
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C. dublinensis subsp. dublinensis
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C. dublinensis subsp. lactaridi
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C. dublinensis subsp. lausannensis
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Legend: + positive 90-100%, - negative 90-100%, [+] positive 75-89%, [-] negative 75-89%, d positive 25-74% of strains
Differential characters between Cronobacter species:
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Enterobacterales, Family Enterobacteriaceae, Genus Cronobacter,
Cronobacter condimenti Joseph et al. 2012.
Old synonym: Enterobacter sakazakii genomospecies 1.
Gram-negative rods. Non-motile. Non-spore-forming.
Colonies on TSA incubated at 37 ºC for 24 hours are 2-3 mm in diameter, opaque,
circular and yellow. Grows on MacConkey agar. In TSB, grows at 45 ºC (optimum
37 ºC), but not at 5 ºC. No haemolysis is observed on sheep blood agar at 37 ºC.
Facultatively anaerobic.
Isolated from spiced meat, water, and a leg infection. Resistant to doxycycline. Sensitive to amikacin, ampicillin, amoxicillin/clavulanic
acid, cefotaxime, cefuroxime, cefpodoxime, ceftazidime, chloramphenicol, ciprofloxacin, gentamicin, imipenem, piperacillin/tazobactam,
trimethoprim, ceftazidime/clavulanic acid, cefotaxime/clavulanic acid, and cefpodoxime/clavulanic acid.
Undetermined.
- Adeolu M, Alnajar S, Naushad S, S Gupta R. Genome-based phylogeny and taxonomy of the 'Enterobacteriales': proposal for
Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov.,
Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Microbiol 2016;
66:5575-5599.
- Iversen C, Mullane N, McCardell B, Tall BD, Lehner A, Fanning S, Stephan R, Joosten H. Cronobacter gen. nov., a new genus to
accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter
malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov.,
Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter
dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int J Syst Evol Microbiol
2008; 58:1442-1447.
- Iversen C, Lehner A, Mullane N, Bidlas E, Cleenwerck I, Marugg J, Fanning S, Stephan R, Joosten H. The taxonomy of Enterobacter
sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter
sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov.,
Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1. BMC Evol Biol 2007; 7:64.
- Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ. Cronobacter condimenti sp. nov., isolated from spiced
meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg
infection, water and food ingredients. Int J Syst Evol Microbiol 2012; 62:1277-1283.
Positive results for arginine dihydrolase, catalase, citrate utilization, DNase (delayed), alpha-glucosidase, beta-galactosidase, gelatin
hydrolysis, indole production, malonate utilization, nitrate reduction, ornithine decarboxylase, Voges-Proskauer test, acid production
from L-arabinose, amygdalin, cellobiose, glucose (without gas production), inositol, mannitol, melibiose, rhamnose, and saccharose.
Can ferment/oxidize L-arabinose, arbutin, cellobiose, esculin, D-fructose, D- and L-fucose, galactose, D-glucose, 10-methyl
alpha-D-glucopyranoside, glycerol, beta-gentiobiose, galacturonic acid, myo-inositol, lactose, maltose, D-mannose, D-mannitol,
melibiose, N-acetylglucosamine, raffinose, L-rhamnose, ribose, salicin, sucrose, trehalose, and D-xylose
Negative results for H2S production, lysine decarboxylase, methyl red test, oxidase, urease, acid production from adonitol, D-sorbitol
and 5-ketogluconate.
No fermentation/oxidation of D- and L-arabitol, D-arabinose, dulcitol, gluconate, 2- and 5-ketogluconate, inositol, lactulose, maltitol,
melezitose, putrescine, cis- or transaconitate, 4-aminobutyrate, palatinose, D-erythritol, adonitol, methyl beta-D-xyloside, L-sorbose,
sorbitol, methyl alpha-D-glucoside, inulin, glycogen, xylitol, D-lyxose, D-tagatose, and turanose.
(c) Costin Stoica