Clostridium saccharolyticum
Cultural characteristics
Biochemical characters
Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster XIVa (non-Clostridium
sensu stricto),
Clostridium saccharolyticum  Murray, Khan and van den Berg 1982.
Gram-negative, spindle-shaped straight rods, 0.5-0.7 x 3.0 µm. Nonmotile; atrichous.
Spores are round, terminal/subterminal and distend the cell; sporulation occurs
readily in chopped-meat carbohydrate broth.
Surface colonies on cellobiose-yeast extract agar after 48 hours incubation are
0.5-1.5 mm in diameter, circular with smooth margins, convex and white; on blood
agar plates, colonies are pinpoint - 1 mm in diameter, circular or slightly irregular, low
convex, with entire or slightly scalloped margins and a mottled or crystalline internal
structure. Cultures in PYG broth have a smooth sediment, with  turbidity, and a pH of
5.1 after incubation for 4 days.
Optimum temperature for growth is 37 ºC. No growth at 14 or 45 ºC. Optimum pH 7.4,
range 6.0-8.8.
Isolated from a methanogenic cellulose-enrichment culture from sewage sludge.
The type strain is susceptible to chloramphenicol, clindamycin, erythromycin, penicillin G, and tetracycline.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Murray W.D., Khan A.W. and van den Berg L., 1982. Clostridium saccharolyticum sp. nov., a saccharolytic species from sewage
    sludge. IJSB vol. 32, no.1, 132-135.
Milk reaction is positive (curd production). Meat is not digested.

Positive results for hydrogen production, ammonia production, esculin hydrolysis, H
2S production, indole production, nitrate reduction,
resazurin reduction, starch hydrolysis, Voges-Proskauer reaction, substrate utilization and/or acid production from: adonitol,
arabinose, cellobiose, esculin, fructose, glucose, glycerol (weak), inositol, lactose, maltose, mannitol, mannose, melezitose,
melibiose, raffinose, rhamnose, ribose, salicin, sorbose, sucrose, trehalose & xylose.

Negative results for catalase, casein hydrolysis, gelatin hydrolysis, lecithinase, lipase, urease, substrate utilization and/or acid
production from: amygdalin, cellulose, dulcitol, erythritol, galactose, glycogen, inulin, sorbitol & starch.
(c) Costin Stoica
Culture media
Biochemical tests
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Abundant growth occurs in PY media supplemented with vitamin K and heme, with or without carbohydrate. There is no growth in a
defined carbohydrate-mineral salt-vitamin medium even when supplemented with synthetic mixtures of amino acids, purines, and
pyrimidines; good growth is obtained in this medium upon addition of yeast extract or in coculture with
Acetivibrio cellulolyticus. In
minimal medium, 0.1% yeast extract, fermentable carbohydrate, iron, and a reduced form of sulfur are required for growth; growth in
this medium is enhanced by addition of B vitamins and phosphate.
Products in PYG broth are acetic, formic, and lactic acids. H
2 and CO2 are produced.