Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Clostridiaceae, Genus Clostridium, Cluster I (Clostridium sensu
stricto), Clostridium colicanis Greetham, Gibson, Giffard, Hippe, Merkhoffer, Steiner, Falsen and Collins 2003.
Gram-negative rods, 0.9-1.0 x 3.0-10.0 µm. Nonmotile. Spores are oval to oblong,
central to subterminal.
Colonies are 3-5 mm in diameter, round, with undulate margin and are slightly convex,
opaque, glossy, and grayish-white. Hemolysis is negative. Anaerobic. Cultures in PYG
broth have a pH of 4.8 after incubation for 6 days.
Growth temperature 30-45 ºC, optimum 37-40 ºC. No growth at 20 or 50 ºC.
Isolated from feces of a male Labrador dog.
- N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
- Greetham H.L., Gibson G.R., Giffard C., Hippe H., Merkhoffer B., Steiner U., Falsen E. and Collins M.D., 2003. Clostridium
colicanis sp. nov., from canine faeces. IJSEM 53, 259-262.
Positive results for esculin hydrolysis, nitrate reduction, starch hydrolysis (weak),
urease, substrate utilized and/or acid produced from: cellobiose, esculin (weak),
fructose, galactose, glucose, lactose, maltose, mannose, ribose, salicin (weak),
starch (weak) & sucrose (weak).
Negative results for casein hydrolysis, catalase, gelatin hydrolysis, indole production, lecithinase, lipase, substrate utilized and/or
acid produced from: amygdalin, arabinose, glycogen, inositol, mannitol, melezitose, melibiose, raffinose, rhamnose, sorbitol,
trehalose & xylose.
Variable results for hippurate hydrolysis.
(c) Costin Stoica