Blautia coccoides
Cultural characteristics
Biochemical characters
Phylum Firmicutes, Class Clostridia, Order Clostridiales, Family Lachnospiraceae, Genus Blautia, Blautia coccoides  (Kaneuchi,
Benno and Mitsouka 1976) Liu, Finegold, Song and Lawson 2008.

Historical synonyms:
Clostridium coccoides  Kaneuchi, Benno and Mitsouka 1976.
Gram-positive, coccoid short rods, 0.6-1.0 x 0.6-1.5 µm, occur in pairs or singly/short
chains. Nonmotile. Spores are round, central/subterminal, slightly swelling the cell;
sporulation occurs most  readily on chopped-meat agar slants or on modified
Eggerth – Gagnon agar plates after incubation at 37 ºC for 2–5 days.
Surface colonies on blood agar plates are pinpoint - 1 mm in diameter, slightly
irregular, slightly undulate, convex, gray-white, shiny, smooth, and nonhemolytic. On
modified Eggerth-Gagnon agar plates, colonies are 1.5-2.5 mm in diameter, convex,
entire, translucent, yellowish gray, smooth, shiny, and nonhaemolytic. Cultures in PYG
broth are turbid with a smooth sediment and have a pH of 4.4 after incubation for 24 h.
Growth is stimulated by fermentable carbohydrates. Abundant gas is detected in PYG
deep agar cultures. Fermentation products in PYG broth cultures are acetic and
succinic acid and hydrogen. Grows at 15 (weak) to 45 ºC; optimum temperature 37 ºC.
Isolated from the cecum of mice fed high-lactose diets and human feces. All strains resist heating at 70 ºC for 10 minutes, but
resistance is variable to 80 ºC. Susceptible to chloramphenicol, clindamycin, erythromycin, and tetracycline. Resistant to penicillin G.
Culture supernatants are nontoxic for mice.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Clostridium Prazmowski 1880. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W.
    Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes,
    Springer, 738-828.
  2. Kaneuchi C., Benno Y. and Mitsouka T., 1976. Clostridium coccoides, a New Species from the Feces of Mice. IJSB 26, 4, 482-486.
  3. Liu, C., Finegold, S.M., Song, Y., and Lawson, P.A., 2008. Reclassification of Clostridium coccoides, Ruminococcus hansenii,
    Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia
    coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov.,
    Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces.
    IJSEM 58, 1896-1902.
Milk reaction is positive (curd). Meat is not digested.

Positive results for hydrogen production (very abundantly), esculin hydrolysis, H
2S production (weak), resazurin reduction, substrate
utilized and/or acid produced from: amygdalin, arabinose, cellobiose, dulcitol, esculin, fructose, galactose, glucose, glycogen, glycerol
(weak), inositol, lactose, maltose, mannitol, mannose, melezitose, melibiose, raffinose, rhamnose, ribose, salicin, sorbitol, sucrose,
trehalose & xylose.

Negative results for casein hydrolysis, catalase, gelatin hydrolysis, indole production, lecithinase, lipase, nitrate reduction, starch
hydrolysis, urease, substrate utilized and/or acid produced from: erythritol, inulin & starch.

Variable and weak results for ammonia, neutral red reduction and Voges-Proskauer test.
(c) Costin Stoica
Culture media
Biochemical tests
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