Phylum Proteobacteria, Class Epsilonproteobacteria, Order Campylobacterales, Family Campylobacteraceae, Genus Campylobacter,
Campylobacter sputorum (Prevot 1940) Veron and Chatelain 1973.Biovars: sputorum, bubulus (doubtful), fecalis and paraureolyticus.
Old synonym: Vibrio sputorum Prevot 1940.
Gram-negative, curved or spiral rods, 0.3-0.5 x 2-4 μm, with rounded ends. Motile by
means of a single flagellum.
Colonies of 3 days-old cultures on blood agar media are 1–2 mm in diameter,
smooth, shiny, low convex, and round. Weak hemolytic activity on blood agar
Growth in broth is light and easily dispersed. Strains may be cultured under
microaerobic or anaerobic gaseous conditions. Anaerobic growth occurs in media
containing fumarate and/or formate.
No growth at 25 ºC. Can grow at 42 ºC. Grows in the presence of 0.05% sodium
fluoride, 2-4% NaCl, 1% glycine, 0,02% safranin, but not in KMnO4 , 2% ox bile and
0.04% triphenyltetrazolium chloride.
Isolated from the human oral cavity, abscesses, skin lesions, the genital tract of bulls, human & animal feces (bovines, sheep, pigs).
- Don J. Brenner and J.J. Farmer III, 2001. Family I. Campylobacteraceae. In: Bergey’s Manual of Systematic Bacteriology, Second
edition,Vol 2, part C, George M. Garrity (Editor-in-Chief), pp 1145-1168.
- S. L. W. On, H. I. Atabay, J. E. L. Corry, C. S. Harrington, and P. Vandamme. Emended description of Campylobacter sputorum and
revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from
cattle and humans. Int J Syst Bacteriol January 1998 48:195-206.
- Notification list. Notification that new names and new combinations have appeared in volume 48, part 1 of the IJSB. Int J Syst
Bacteriol 1998; 48:629. https://www.microbiologyresearch.org/docserver/fulltext/ijsem/48/2/ijs-48-2-629.pdf?
Positive results for selenite reduction, nitrate reduction, oxidase, and H2S production on TSI.
Negative results for indoxyl acetate hydrolysis, alkaline phosphatase, and hippurate hydrolysis. No carbohydrates fermentation.
Biochemical differences: biovar sputorum - catalase and urease negative; biovar faecalis - catalase positive and urease negative
strains, biovar paraureolyticus - catalase negative and urease positive. Biovar bubulus cannot be differentiated from biovar
sputorum and has a questionable validity. According to On et al. strains classified previously as biovar bubulus should be reclassified
as biovar sputorum.
(c) Costin Stoica