Phylum “Actinobacteria”, Class Actinobacteria, Order Bifidobacteriales, Family Bifidobacteriaceae, Genus Bifidobacterium,
Bifidobacterium mongoliense Watanabe, Makino, Sasamoto, Kudo, Fujimoto and Demberel 2009.
Gram-positive rods of various shapes, 0.4-0.6 / 0.8-2.0 µm, with rounded or tapered
ends, sometimes curved, swollen and branched. Non-motile. Non-spore-forming.
Colonies are white, convex, opaque, smooth, 2-3 mm in diameter on modified MRS
agar (glucose replaced with 1 % lactose) supplemented with 0.001 % sodium azide
and 0.001 % cycloheximide and incubated anaerobically at 30 ºC for 5 days; pinpoint
colonies (0.2–0.4 mm in diameter) are formed under aerobic conditions after 2 days
incubation. Growth temperature 15-35 ºC, optimum 25-30 ºC; no growth at 10 or 40
ºC. pH range is 4.5-8.0; optimum pH 6.5-7.0. Facultatively anaerobic.
Isolated from airag, a traditional fermented mare milk from Mongolia.
- Watanabe K, Makino H, Sasamoto M, Kudo Y, Fujimoto J and Demberel S 2009. Bifidobacterium moongoliense sp. nov., from airag,
a traditional fermented mare’s milk from Mongolia . Int J Syst Evol Microbiol 59, 1535-1540.
Description is based mostly on API 50 CHL results
Positive results for esculin hydrolysis, acid production from L-arabinose, D-galactose, D-glucose, glycogen, methyl alpha-D-
glucopyranoside, lactose, maltose, melibiose, raffinose, starch, sucrose and turanose.
Negative results for catalase, oxidase, acid production from D-adonitol, D- and L-arabitol, D-arabinose, dulcitol, erythritol, esculin, D-
and L-fucose, D-fructose, glycerol, N-acetylglucosamine, inulin, inositol, 2- and 5-ketogluconate, D-lyxose, D-mannitol, D-mannose,
melezitose, methyl alpha-D-mannopyranoside, rhamnose, D-sorbitol, L-sorbose, tagatose, trehalose, methyl beta-D-xylopyranoside,
xylitol, and D-xylose.
Variable results for acid production from amygdalin, arbutin, cellobiose, gluconate, gentiobiose, D-ribose and salicin.
(c) Costin Stoica