Phylum “Actinobacteria”, Class Actinobacteria, Order Bifidobacteriales, Family Bifidobacteriaceae, Genus Bifidobacterium,
Bifidobacterium actinocoloniiforme Killer, Kopecny, Mrazek, Koppova, Havlik, Benada and Kott 2011.
Gram-positive irregularly shaped rods, 0.3-0.6 x 0.4-1.2 µm, with enlarged or tapered
ends, occurring singly or in short chains. Non-motile. Non-spore-forming.
Colonies are cream in colour, sometimes irregularly circular with entire edges and
rigid cores and 2.0-3,9 mm in diameter after 2 days of incubation under anaerobic
conditions on TPY agar; some colonies have filamentous parts growing around the
solid core after 72 hours of incubation. The colonies reach 1.7-2.6 mm after 2 days of
incubation under microaerophilic conditions. Growth temperature 25-37 ºC,no growth
at 47 ºC. Grows at pH 5.0.
Isolated from the digestive tract contents of a bumblebee (Bombus lucorum), Czech Republic.
- Killer J, Kopecny J, Mrazek J, Koppova I, Havlik J, Benada O and Kott T, 2011. Bifidobacterium actinocoloniiforme sp. nov. and
Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract. Int J Syst Evol Microbiol 61, 1315-1321.
Description is based mostly on API 50 CHL and API Rapid ID 32A results.
Positive results for arginine arylamidase, alanine arylamidase, alpha- and beta-galactosidase, alpha- and beta-glucosidase, histidine
arylamidase, leucine arylamidase, phenylalanine arylamidase, proline arylamidase, tyrosine arylamidase, acid production from
amygdalin, arbutin, L-arabinose, cellobiose (weak), D-galactose, D-glucose, melibiose (weak), D-ribose, salicin, sucrose, trehalose,
Negative results for alkaline phosphatase, arginine dihydrolase, catalase, alpha-fucosidase, beta-glucuronidase, indole production,
nitrate reduction, N-acetyl-beta-glucosaminidase, oxidase, pyroglutamic acid arylamidase, urease, acid production from D-adonitol, D-
and L-arabitol, dulcitol, erythritol, D- and L-fucose, D-fructose, gentiobiose, gluconate, glycerol, inulin, inositol, 2- and 5-ketogluconate,
D-lyxose, maltose, D-mannitol, D-mannose, melezitose, methyl alpha-D-glucopyranoside, methyl alpha-D-mannopyranoside, methyl
beta-D-xylopyranoside, N-acetylglucosamine, raffinose, rhamnose, D-sorbitol, L-sorbose, starch, tagatose, and xylitol.
(c) Costin Stoica