Lysinibacillus sphaericus
Lysinibacillus sphaericus - Gram stain
Lysinibacillus sphaericus colonies on Blood Agar
Cultural characterisics
Biochemical characters
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Planococcaceae, Genus Lysinibacillus, Lysinibacillus sphaericus  (Meyer
and Neide 1904) Ahmed, Yokota, Yamazoe and Fujiwara 2007.

Historical synonyms:
Bacillus sphaericus Neide (1904),  B. lactimorbi Jordan  and
Harris (1908),
B. loehnisii Gibson (1935b), B. rotans Roberts (1935), B. sphaericus
var. rotans
Smith, Gordon and Clarck (1946), B. serositidis Lacorte (1932).
Gram-positive or Gram-variable, 1.5-5.0 x 0.6-1.0 µm, motile by fairly abundant  
peritrichous flagella or only polar flagella that coalesced in a long ribbon.
Spores are spherical, subterminal or terminal, swelling the sporangia.
Colonies are opaque, unpigmented, smooth, often glossy. Growth on nutrient agar
varies in different strains from compact and heaped to a wide spreading over the
surface; uncommon strains produce pink colonies. Maximum temperature 30-45 ºC;
minimum temperature 5-15 ºC. Grow at ph 5.7-9.5. Growth at 5.7-6 pH is a variable
property. Aerobic. Grow in 0-5% NaCl. Nonhemolytic.
Biotin and thiamin are required for growth; allantoin or urate are not required.
Isolated from food, water, clinical specimens and mosquitoes. Spores occur in soil.
Resistant to chloramphenicol (8 µg/ml), erythromicyn (1 µg/ml), streptomycin (8 µg/ml),
tetracycline (2 µg/ml) and rifampicin (0.25 µg/ml).
Is an accidental human pathogen; may be involved in bacteremia, meningitis, pseudotumors, food infections.
  1. Gordon R.E., Haynes W.C., Pang C.H. (1973) – The genus Bacillus . Agriculture Handbook No. 427, U.S.D.A., Washington D.C.
  2. Buchanan R.E., Gibbons N.E., Cowan S.T., Holt J.G., Liston J., Murray R.G.E., Niven C.F., Ravin A.W., Stanier R.W. ( 1974) –  
    Bergey’s Manual of Determinative Bacteriology, Eight Edition, The Williams & Wilkins Company, Baltimore.
  3. Buiuc D., Negut M. , 1999. Tratat de Microbiologie Clinica, Editura Medicala, Bucuresti.
  4. N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
    Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  5. Ahmed I.,Yokota A, Yamazoe A.,and Fujiwara T., 2007. Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of
    Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. IJSEM
    57, 1117-1126.
Positive results for deamination of phenylalanine, oxidase, hydrolysis of casein, citrate
utilization & hydrolysis of Tween 20.

Negative results for arginine dihydrolase, degradation of tyrosine, beta-galactosidase,
lysine decarboxylase, ornithine decarboxylase, hydrolysis of esculin, hydrolysis of
hippurate, egg yolk reaction, indole production, H
2S production, acid production from:
D-mannose, methyl beta-xyloside, glycerol, glycogen, salicin, starch, adonitol,
amygdalin, D- or L-arabitol, arbutin, cellobiose, dulcitol, erythritol, fructose, D- or
L-fucose, galactose, beta-gentibiose, gluconate, meso-inositol, inulin, 2- or
5-ketogluconate, lactose, lyxose, maltose,  melezitose, melibiose, raffinose, rhamnose,
ribose, sorbitol, sorbose, sucrose, trehalose, xylitol & D- or L-xylose.

Variable results for hydrolysis of urea, hydrolysis of Tween 80, hydrolysis of gelatin &
acid production from N-acetyl-D-glucosamine.
(c) Costin Stoica
Culture media
Biochemical tests
Previous page