Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus horneckiae Vaishampayan, Probst,
Krishnamurthi, Ghosh, Osman, McDowall, Ruckmani, Mayilraj and Venkateswaran, 2010.
Gram-positive, motile rods, 1.0-1.5 x 4.0-6.0 µm. Produce subterminal, oval
endospores which exhibit an external layer that appeared to attract other spores and
result in the formation of a biofilm or aggregates.
Colonies are yellowish, plain, 3 mm in diameter, with irregular edges after 24 hours
on TSA at 32 ºC. No pigment production. Aerobic; no anaerobic growth. Grow at pH
6-10 (optimum pH 7) and in 0-10% NaCl media. Temperature range 5-40 ºC.
Isolated from a clean room of the Kennedy Space Center. Spores were resistant to UV radiation up to 1000 J/m2.
- Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S. and Venkateswaran K.,
2010. Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. IJSEM 60, 1031-1037.
Positive results for catalase, hydrolysis of gelatin (weak reaction) and nitrate reduction.
Negative results for arginine dihydrolase, beta-galactosidase, hydrolysis of esculin,
hydrolysis of urea, oxidase, indole production, acid production from: D- or L-arabinose, N-acetyl-D-glucosamine, adonitol, amygdalin,
D- or L-arabitol, arbutin, cellobiose, dulcitol, erythritol, fructose, D- or L-fucose, galactose, beta-gentiobiose, gluconate, D-glucose,
glycerol, glycogen, meso-inositol, inulin, 2- or 5-ketogluconate, lactose, lyxose, D-mannitol, D-mannose, maltose, melezitose,
melibiose, raffinose, rhamnose, ribose, salicin, starch, sorbitol, sorbose, sucrose, trehalose, xylitol, D- or L-xylose.
(c) Costin Stoica