Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus cohnii Spanka and Fritze 1993.
Gram-positive, peritrichously motile rods, 0.6-0.7 µm cell width, subterminally to
terminally, ellipsoidal endospores in swollen sporangia.
Colonies are creamy-white on alkaline meat extract-peptone medium, 1-2 mm in
diameter (2 days at 45 ºC). Obligately alkaliphilic. Grow at pH 9-10 ( 9.7 optimum).
Grows in 2 and 5% NaCl. Growth temperature 10-47 ºC. Allantoin, urate or NaCl are
not required for growth.
Isolated from soil and feces (of old horses), Denmark and Germany; one strain was contaminant on an agar plate in Great Britain.
Unknown.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- Spanka R. and Fritze D., 1993. Bacillus cohnii sp. nov. , a New, Obligately Alkaliphilic, Oval-Spore-Forming Bacillus Species with
Ornithine and Aspartic Acid Instead of Diaminopimelic Acid in the Cell Wall. IJSB 43, 1, 150-156.
Positive results for beta-galactosidase, hydrolysis of hippurate, hydrolysis of gelatin,
oxidase, catalase, nitrate reduction, hydrolysis of casein, hydrolysis of starch,
hydrolysis of Tween 20, 60 and 80.
Negative results for deamination of phenylalanine and hydrolysis of urea.
Strain didn't grow when testing Voges-Proskauer reaction, acid production from: L-arabinose, D-glucose, D-mannitol, D-mannose,
methyl beta-xyloside, glycerol, glycogen, salicin, starch and D-xylose.
(c) Costin Stoica